ZAP1 Antibody

Code CSB-PA342851XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ZAP1 Polyclonal antibody
Uniprot No.
Target Names
ZAP1
Alternative Names
ZAP1 antibody; YJL056C antibody; J1145 antibody; Zinc-responsive transcriptional regulator ZAP1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ZAP1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription regulator controlling zinc-responsive gene expression. Binds to zinc-responsive elements (ZREs) (consensus sequence 5'-ACCYYNAAGGT-3') in the promoter of target genes. Recruits SWI/SNF, SAGA, and Mediator complexes as coactivators in a zinc-responsive manner. Involved in zinc ion homeostasis by zinc-responsive transcriptional regulation of the zinc uptake system genes ZTR1 and ZTR2. Positively regulates ETR1 expression, affecting mitochondrial function.
Gene References into Functions
  1. In this study,the authors performed the first phylogenetic classification of shochu strains (Saccharomyces cerevisiae strains) using the nucleotide sequences of genes that reflect genome-level phylogeny. The single-nucleotide polymorphisms information identified in the present study is expected to be useful for distinguishing shochu yeast strains as part of quality control at shochu breweries PMID: 28703391
  2. Results suggest that the Zap1 regulon includes ZRG17; Zap1-directed transcriptional regulation has biological importance in maintaining ER function and zinc homeostasis. PMID: 21250939
  3. Roles of two activation domains in Zap1 in the response to zinc deficiency in Saccharomyces cerevisiae. PMID: 21177862
  4. AD1 (activation domain) residues 332-402 is critical for control of gene expression in response to zinc PMID: 16045625
  5. results describe the solution structure of two zinc fingers (zf1-2(CA3)) derived from a zinc-responsive domain of Zap1 (zf1-2) PMID: 16483601
  6. results indicate that the kinetic lability of ZF1/2 in vivo is a key component of Zap1 zinc responsiveness PMID: 16720702
  7. During zinc deficiency, Zap1 mediates the repression of two of the most abundant zinc-requiring enzymes. PMID: 17139254
  8. through the differential regulation of its target genes, Zap1 prioritizes mechanisms of zinc homeostasis and adaptive responses to zinc deficiency PMID: 18673560

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Subcellular Location
Nucleus.
Database Links

KEGG: sce:YJL056C

STRING: 4932.YJL056C

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