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Recombinant Saccharomyces cerevisiae Zinc-responsive transcriptional regulator ZAP1 (ZAP1), partial

Recombinant Saccharomyces cerevisiae Zinc-responsive transcriptional regulator ZAP1 (ZAP1), partial

Code	CSB-YP342851SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP342851SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP342851SVG-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP342851SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP342851SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

ZAP1

Uniprot No.

P47043

Alternative Names

ZAP1; YJL056C; J1145; Zinc-responsive transcriptional regulator ZAP1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Transcription regulator controlling zinc-responsive gene expression. Binds to zinc-responsive elements (ZREs) (consensus sequence 5'-ACCYYNAAGGT-3') in the promoter of target genes. Recruits SWI/SNF, SAGA, and Mediator complexes as coactivators in a zinc-responsive manner. Involved in zinc ion homeostasis by zinc-responsive transcriptional regulation of the zinc uptake system genes ZTR1 and ZTR2. Positively regulates ETR1 expression, affecting mitochondrial function.

Gene References into Functions

In this study,the authors performed the first phylogenetic classification of shochu strains (Saccharomyces cerevisiae strains) using the nucleotide sequences of genes that reflect genome-level phylogeny. The single-nucleotide polymorphisms information identified in the present study is expected to be useful for distinguishing shochu yeast strains as part of quality control at shochu breweries PMID: 28703391
Results suggest that the Zap1 regulon includes ZRG17; Zap1-directed transcriptional regulation has biological importance in maintaining ER function and zinc homeostasis. PMID: 21250939
Roles of two activation domains in Zap1 in the response to zinc deficiency in Saccharomyces cerevisiae. PMID: 21177862
AD1 (activation domain) residues 332-402 is critical for control of gene expression in response to zinc PMID: 16045625
results describe the solution structure of two zinc fingers (zf1-2(CA3)) derived from a zinc-responsive domain of Zap1 (zf1-2) PMID: 16483601
results indicate that the kinetic lability of ZF1/2 in vivo is a key component of Zap1 zinc responsiveness PMID: 16720702
During zinc deficiency, Zap1 mediates the repression of two of the most abundant zinc-requiring enzymes. PMID: 17139254
through the differential regulation of its target genes, Zap1 prioritizes mechanisms of zinc homeostasis and adaptive responses to zinc deficiency PMID: 18673560