Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ZEP Polyclonal antibody

Uniprot No.

Q9FGC7

Target Names

ZEP

Alternative Names

ZEP antibody; ABA1 antibody; IBS3 antibody; LOS6 antibody; NPQ2 antibody; At5g67030 antibody; K8A10.10 antibody; Zeaxanthin epoxidase antibody; chloroplastic antibody; AtZEP antibody; EC 1.14.15.21 antibody; Protein ABA DEFICIENT 1 antibody; AtABA1 antibody; Protein IMPAIRED IN BABA-INDUCED STERILITY 3 antibody; Protein LOW EXPRESSION OF OSMOTIC STRESS-RESPONSIVE GENES 6 antibody; Protein NON-PHOTOCHEMICAL QUENCHING 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ZEP protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Zeaxanthin epoxidase that plays an important role in the xanthophyll cycle and abscisic acid (ABA) biosynthesis. Converts zeaxanthin into antheraxanthin and subsequently violaxanthin. Required for resistance to osmotic and drought stresses, ABA-dependent stomatal closure, seed development and dormancy, modulation of defense gene expression and disease resistance and non-photochemical quencing (NPQ). Through its role in ABA biosynthesis, regulates the expression of stress-responsive genes such as RD29A during osmotic stress and is required for normal plant growth during vegetative development. Is required for late skotomorphogenic growth through its role in the xanthophyll carotenoids neoxanthin, violaxanthin and antheraxanthin biosynthesis. Required for beta-aminobutyric acid (BABA)-induced priming in disease resistance, tolerance to salt and drought stresses and sterility. Participates in NPQ by regulating the level of zeaxanthin in photosynthetic energy conversion. NPQ is a process that maintains the balance between dissipation and utilization of light energy to minimize the generation of oxidizing molecules and the molecular damages they can generate.

Gene References into Functions

ZEP role in the carotenoid degradation in maturing seed.ZEP is expressed in the haplotype specific fashion during late seed development. PMID: 27208224
aba1 may serve as an alternative model to other (1)singlet oxygen (O2-)overproducing mutants of Arabidopsis for investigating (1)O2-mediated cell death. PMID: 28709027
These data support a tissue- and stress-specific accumulation of the ZEP protein in accordance with its different functions in abscissic acid biosynthesis and the xanthophyll cycle. PMID: 25416291
A description of the molecular and phenotypic characterization of a large series of mutant alleles of the ABA1 gene, which cause different degrees of ABA deficiency, four of them previously isolated and the remaining five novel, is presented. PMID: 15983017
ABA1 is required for skotomorphogenic growth, and beta-carotene-derived xanthophylls are putative regulators of skotomorphogenesis. PMID: 17996011
AtZEP plays important roles in response to osmotic stress. PMID: 18680727

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Subcellular Location

Plastid, chloroplast.

Tissue Specificity

Expressed in leaves, stems and flowers, and at lower levels in roots and siliques.

Database Links

KEGG: ath:AT5G67030

STRING: 3702.AT5G67030.1

UniGene: At.23748

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ZEP Antibody

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