Recombinant Arabidopsis thaliana Zeaxanthin epoxidase, chloroplastic (ZEP), partial

Code CSB-YP884229DOA
MSDS
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Source Yeast
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Code CSB-EP884229DOA
MSDS
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Source E.coli
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Code CSB-EP884229DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884229DOA
MSDS
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Source Baculovirus
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Code CSB-MP884229DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ZEP
Uniprot No.
Alternative Names
ZEP; ABA1; IBS3; LOS6; NPQ2; At5g67030; K8A10.10; Zeaxanthin epoxidase; chloroplastic; AtZEP; EC 1.14.15.21; Protein ABA DEFICIENT 1; AtABA1; Protein IMPAIRED IN BABA-INDUCED STERILITY 3; Protein LOW EXPRESSION OF OSMOTIC STRESS-RESPONSIVE GENES 6; Protein NON-PHOTOCHEMICAL QUENCHING 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Zeaxanthin epoxidase that plays an important role in the xanthophyll cycle and abscisic acid (ABA) biosynthesis. Converts zeaxanthin into antheraxanthin and subsequently violaxanthin. Required for resistance to osmotic and drought stresses, ABA-dependent stomatal closure, seed development and dormancy, modulation of defense gene expression and disease resistance and non-photochemical quencing (NPQ). Through its role in ABA biosynthesis, regulates the expression of stress-responsive genes such as RD29A during osmotic stress and is required for normal plant growth during vegetative development. Is required for late skotomorphogenic growth through its role in the xanthophyll carotenoids neoxanthin, violaxanthin and antheraxanthin biosynthesis. Required for beta-aminobutyric acid (BABA)-induced priming in disease resistance, tolerance to salt and drought stresses and sterility. Participates in NPQ by regulating the level of zeaxanthin in photosynthetic energy conversion. NPQ is a process that maintains the balance between dissipation and utilization of light energy to minimize the generation of oxidizing molecules and the molecular damages they can generate.
Gene References into Functions
  1. ZEP role in the carotenoid degradation in maturing seed.ZEP is expressed in the haplotype specific fashion during late seed development. PMID: 27208224
  2. aba1 may serve as an alternative model to other (1)singlet oxygen (O2-)overproducing mutants of Arabidopsis for investigating (1)O2-mediated cell death. PMID: 28709027
  3. These data support a tissue- and stress-specific accumulation of the ZEP protein in accordance with its different functions in abscissic acid biosynthesis and the xanthophyll cycle. PMID: 25416291
  4. A description of the molecular and phenotypic characterization of a large series of mutant alleles of the ABA1 gene, which cause different degrees of ABA deficiency, four of them previously isolated and the remaining five novel, is presented. PMID: 15983017
  5. ABA1 is required for skotomorphogenic growth, and beta-carotene-derived xanthophylls are putative regulators of skotomorphogenesis. PMID: 17996011
  6. AtZEP plays important roles in response to osmotic stress. PMID: 18680727

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Subcellular Location
Plastid, chloroplast.
Tissue Specificity
Expressed in leaves, stems and flowers, and at lower levels in roots and siliques.
Database Links

KEGG: ath:AT5G67030

STRING: 3702.AT5G67030.1

UniGene: At.23748

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