z Antibody

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Code	CSB-PA357816XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) z Polyclonal antibody

Uniprot No.

P09956

Target Names

Alternative Names

z antibody; CG7803 antibody; Regulatory protein zeste antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) z protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Involved in transvection phenomena (= synapsis-dependent gene expression), where the synaptic pairing of chromosomes carrying genes with which zeste interacts influences the expression of these genes. Zeste binds to DNA and stimulates transcription from a nearby promoter.

Gene References into Functions

This study found that knockdown of zeste (z), a gene encoding a sequence-specific DNA-binding protein implicated in transvection, in salivary gland cells advances the initiation of their degeneration PMID: 26577029
the crystal structure of the DNA binding domain of Zeste in complex with a 19-bp DNA duplex containing the consensus recognition sequence motif. PMID: 26478222
Study provides additional evidence that Zeste protein plays no role in the activation of the white gene transcription; does not rule out that the white gene enhancer is able to recruit RNAPII and function as a weak promoter PMID: 25937222
Polycomb group (PcG) component, Enhancer of Zeste [E(z)], a histone transferase that generates trimethylation at lysine 27 of histone H3, maintains germline identity in Drosophila adult testes PMID: 24675960
Zeste mediates Polycomb repression PMID: 11880343
the Zeste-DNA backbone contacts all map to one face of the DNA PMID: 12354778
interaction of zeste response elements do not block yellow enhancers PMID: 14531075
DNA-binding sites for the Zeste protein within cellular memory modules are necessary for the inheritance of active chromatin through Zeste-dependent recruitment of BRM PMID: 14963490
Zeste is also required for insulator bypass by the eye enhancer. Taken together, these results show that Zeste can support specific long-range interactions between enhancers and promoters. PMID: 19578867

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Subcellular Location

Nucleus.

Database Links

KEGG: dme:Dmel_CG7803

STRING: 7227.FBpp0303588

UniGene: Dm.41

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