Recombinant Drosophila melanogaster Regulatory protein zeste (z), partial

Code CSB-YP357816DLU
MSDS
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Source Yeast
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Code CSB-EP357816DLU
MSDS
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Source E.coli
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Code CSB-EP357816DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP357816DLU
MSDS
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Source Baculovirus
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Code CSB-MP357816DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
z
Uniprot No.
Alternative Names
z; CG7803; Regulatory protein zeste
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in transvection phenomena (= synapsis-dependent gene expression), where the synaptic pairing of chromosomes carrying genes with which zeste interacts influences the expression of these genes. Zeste binds to DNA and stimulates transcription from a nearby promoter.
Gene References into Functions
  1. This study found that knockdown of zeste (z), a gene encoding a sequence-specific DNA-binding protein implicated in transvection, in salivary gland cells advances the initiation of their degeneration PMID: 26577029
  2. the crystal structure of the DNA binding domain of Zeste in complex with a 19-bp DNA duplex containing the consensus recognition sequence motif. PMID: 26478222
  3. Study provides additional evidence that Zeste protein plays no role in the activation of the white gene transcription; does not rule out that the white gene enhancer is able to recruit RNAPII and function as a weak promoter PMID: 25937222
  4. Polycomb group (PcG) component, Enhancer of Zeste [E(z)], a histone transferase that generates trimethylation at lysine 27 of histone H3, maintains germline identity in Drosophila adult testes PMID: 24675960
  5. Zeste mediates Polycomb repression PMID: 11880343
  6. the Zeste-DNA backbone contacts all map to one face of the DNA PMID: 12354778
  7. interaction of zeste response elements do not block yellow enhancers PMID: 14531075
  8. DNA-binding sites for the Zeste protein within cellular memory modules are necessary for the inheritance of active chromatin through Zeste-dependent recruitment of BRM PMID: 14963490
  9. Zeste is also required for insulator bypass by the eye enhancer. Taken together, these results show that Zeste can support specific long-range interactions between enhancers and promoters. PMID: 19578867

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Subcellular Location
Nucleus.
Database Links

KEGG: dme:Dmel_CG7803

STRING: 7227.FBpp0303588

UniGene: Dm.41

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