zfh1 Antibody

Code CSB-PA335354XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) zfh1 Polyclonal antibody
Uniprot No.
Target Names
zfh1
Alternative Names
zfh1 antibody; zfh-1 antibody; CG1322 antibody; Zinc finger protein 1 antibody; Zinc finger homeodomain protein 1 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) zfh1 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the development of the embryonic central nervous system, embryonic mesoderm and adult musculature.
Gene References into Functions
  1. Zfh1 binds and downregulates salvador and kibra, two tumour suppressor genes of the Hippo/Wts/Yki pathway, thereby restricting Yki activation and proliferation to the Zfh1(+) stem cells. PMID: 30002131
  2. Zfh1 levels are increased in somatic cells that contact germ cells, and Woc is required for a Stat92E-mediated upregulation of zfh1 transcription. PMID: 24903753
  3. zfh1 is an important regulator of the immune response in Drosophila. PMID: 23178405
  4. Zfh1 is required for ventral motor axon exit from the CNS. PMID: 16458285
  5. Our observations on the regulation of Zfh-1 provide a new approach for examining the function of Zfh-1 in motor neurons and larval locomotion. PMID: 17702602
  6. Study shows that Zfh1 cell autonomously regulates motoneuronal outgrowth and larval growth of neuromuscular junctions and that the growth of NMJs is dependent on the dosage of Zfh1. PMID: 18499094
  7. Zfh-1 is a presumptive somatic target of Jak/STAT signaling, demonstrating that it is necessary and sufficient to maintain somatic stem cells. PMID: 18593558
  8. Study shows a novel function for Zfh1 as an anti-apoptotic molecule and uncovers a cryptic JNK-dependent apoptotic programme in ePG10, which is normally blocked by Zfh1. PMID: 19745814

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Subcellular Location
Nucleus.
Protein Families
Delta-EF1/ZFH-1 C2H2-type zinc-finger family
Tissue Specificity
Mesoderm and mesodermally-derived structures in the embryo including the dorsal vessel, support cells of the gonads, and segment-specific arrays of adult muscle precursor. Also identified in motor neurons of developing CNS.
Database Links

KEGG: dme:Dmel_CG1322

STRING: 7227.FBpp0303607

UniGene: Dm.4708

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