Recombinant Drosophila melanogaster Zinc finger protein 1 (zfh1), partial

Code CSB-YP335354DLU
MSDS
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Source Yeast
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Code CSB-EP335354DLU
MSDS
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Source E.coli
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Code CSB-EP335354DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP335354DLU
MSDS
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Source Baculovirus
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Code CSB-MP335354DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
zfh1
Uniprot No.
Alternative Names
zfh1; zfh-1; CG1322; Zinc finger protein 1; Zinc finger homeodomain protein 1
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the development of the embryonic central nervous system, embryonic mesoderm and adult musculature.
Gene References into Functions
  1. Zfh1 binds and downregulates salvador and kibra, two tumour suppressor genes of the Hippo/Wts/Yki pathway, thereby restricting Yki activation and proliferation to the Zfh1(+) stem cells. PMID: 30002131
  2. Zfh1 levels are increased in somatic cells that contact germ cells, and Woc is required for a Stat92E-mediated upregulation of zfh1 transcription. PMID: 24903753
  3. zfh1 is an important regulator of the immune response in Drosophila. PMID: 23178405
  4. Zfh1 is required for ventral motor axon exit from the CNS. PMID: 16458285
  5. Our observations on the regulation of Zfh-1 provide a new approach for examining the function of Zfh-1 in motor neurons and larval locomotion. PMID: 17702602
  6. Study shows that Zfh1 cell autonomously regulates motoneuronal outgrowth and larval growth of neuromuscular junctions and that the growth of NMJs is dependent on the dosage of Zfh1. PMID: 18499094
  7. Zfh-1 is a presumptive somatic target of Jak/STAT signaling, demonstrating that it is necessary and sufficient to maintain somatic stem cells. PMID: 18593558
  8. Study shows a novel function for Zfh1 as an anti-apoptotic molecule and uncovers a cryptic JNK-dependent apoptotic programme in ePG10, which is normally blocked by Zfh1. PMID: 19745814

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Subcellular Location
Nucleus.
Protein Families
Delta-EF1/ZFH-1 C2H2-type zinc-finger family
Tissue Specificity
Mesoderm and mesodermally-derived structures in the embryo including the dorsal vessel, support cells of the gonads, and segment-specific arrays of adult muscle precursor. Also identified in motor neurons of developing CNS.
Database Links

KEGG: dme:Dmel_CG1322

STRING: 7227.FBpp0303607

UniGene: Dm.4708

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