Bovine α1-Acid glycoprotein,α1-AGP ELISA Kit

Instructions
Code CSB-E08579b
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name orosomucoid 1
Alternative Names ORM1 ELISA kit; AGPAlpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
Abbreviation ORM1
Uniprot No. Q3SZR3
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 ng/mL-4000 ng/mL
Sensitivity 15.6 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 102  
Range % 94-106  
1:2000 Average % 92  
Range % 84-97  
1:4000 Average % 101  
Range % 95-104  
1:8000 Average % 97  
Range % 92-101  
Recovery
The recovery of bovine α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 88-99  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
4000 0.229 0.225 0.227    
2000 0.450 0.459 0.455    
1000 0.698 0.656 0.677    
500 1.016 0.987 1.002    
250 1.264 1.243 1.254    
125 1.572 1.514 1.543    
62.5 1.671 1.634 1.653    
0 1.815 1.789 1.802    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain (By similarity). Appears to function in modulating the activity of the immune system during the acute-phase reaction (By similarity).
Gene References into Functions
  1. The presence of lipopolysaccharide binding protein (LBP) and alpha1-acid glycoprotein (AGP) was demonstrated in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines. PMID: 25542063
  2. results provide evidence that locally produced alpha-1 acid glycoprotein may be involved in protecting sperm from phagocytosis by polymorphonuclear neutrophils in the bovine oviduct PMID: 24931131
  3. Studied the regulation and immune function of AGP in cultured bovine oviduct epithelial cells. PMID: 25123565
  4. As in human AGP and several other lipocalins, a temperature-induced molten globule state has been observed in the denaturation pathway of bovine AGP. PMID: 24530968
  5. In the mitochondrial membrane, both AGP and MLQ proteins are almost quantitatively bound to ATP synthase. PMID: 17575325
  6. Alpha1-acid glycoprotein isoforms that can be detected in bovine milk are reported. PMID: 17692138
  7. study provides evidence that the expression of CD18 was downregulated by the plasma glycoforms of alpha-1 acid glycoprotein and inhibited the chemotaxis of monocytes PMID: 18577362

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Subcellular Location Secreted
Protein Families Calycin superfamily, Lipocalin family
Database Links

KEGG: bta:497200

STRING: 9913.ENSBTAP00000022991

UniGene: Bt.49064

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