Bovine Deoxyribonuclease-1(DNASE1) ELISA kit

Code CSB-EL007049BO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
deoxyribonuclease I
Alternative Names
DNASE1 ELISA Kit; DNL1Deoxyribonuclease-1 ELISA Kit; EC 3.1.21.1 ELISA Kit; Deoxyribonuclease I ELISA Kit; DNase I ELISA Kit
Abbreviation
DNASE1
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
3.1 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine DNASE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:20 Average % 85
Range % 80-89
1:40 Average % 94
Range % 90-98
1:80 Average % 103
Range % 99-109
1:160 Average % 97
Range % 92-101
Recovery
The recovery of bovine DNASE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 84-92
EDTA plasma (n=4) 93 89-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
800 2.399 2.416 2.408 2.273
400 2.213 2.195 2.204 2.069
200 1.813 1.741 1.777 1.642
100 1.204 1.227 1.216 1.081
50 0.683 0.713 0.698 0.563
25 0.400 0.392 0.396 0.261
12.5 0.292 0.284 0.288 0.153
0 0.131 0.138 0.135  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine DNASE1 ELISA Kit was designed for the quantitative measurement of Bovine DNASE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.1 pg/mL.

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Target Background

Function
(From Uniprot)
Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
Gene References into Functions
  1. ascorbic acid has the ability to inhibit DNase I, one of the main endonucleases involved in DNA fragmentation during apoptosis. PMID: 29551685
  2. the practical capability of this assay system was successfully demonstrated by its use to determine DNase I activity in bovine urine. PMID: 27570054
  3. Specificity in DNA-DNase I interaction is mediated by DNA flexibility, which influences the induced-fit transitions required to form productive complexes. PMID: 19850052
  4. results indicated that the backbone hydrogen bonding in the antiparallel beta-sheet formed by its N- and C-termini, rather than the side-chain interactions, is crucial for the correct protein folding PMID: 15311926
Subcellular Location
Secreted. Zymogen granule. Nucleus envelope.
Protein Families
DNase I family
Database Links
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