Code | CSB-E08585b |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of bovine Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1000 | Average % | 84 | ||||
Range % | 80-88 | |||||
1:2000 | Average % | 90 | ||||
Range % | 85-94 | |||||
1:4000 | Average % | 103 | ||||
Range % | 99-112 | |||||
1:8000 | Average % | 98 | ||||
Range % | 94-102 |
The recovery of bovine Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 90-99 | ||||
EDTA plasma (n=4) | 92 | 88-96 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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The bovine haptoglobin (HP) ELISA Kit is engineered for accurate measurement of bovine haptoglobin levels from samples including serum or plasma. It uses the cmpetitive-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the bovine haptoglobin content in the sample. The color intensity is negatively correlated with haptoglobin content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
Haptoglobin is an acute-phase protein that is synthesized mainly in the liver and lungs and is crucial for the elimination of free hemoglobin and the neutralization of oxidative damage. It has powerful regulatory activity on lymphocytes. Additionally, haptoglobin exhibits immunoregulatory properties, participates in angiogenesis and inhibition of nitric oxide, stimulates tissue repair, etc.
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a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?