Bovine Haptoglobin,Hpt/HP ELISA KIT

Code CSB-E08585b
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q2TBU0
Lead Time 3-5 working days
Abbreviation HP
Target Name haptoglobin
Alias BP, HP2-ALPHA-2, HPA1S, MGC111141, binding peptide|haptoglobin alpha(1S)-beta|haptoglobin alpha(2FS)-beta|haptoglobin, alpha polypeptide|haptoglobin, beta polypeptide
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 7.81 ng/mL-2000 ng/mL
Sensitivity 7.81 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn s disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson s disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene.
HGNC 16937
RGD 708572
MGI 1929501
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 84  
Range % 80-88  
1:2000 Average % 90  
Range % 85-94  
1:4000 Average % 103  
Range % 99-112  
1:8000 Average % 98  
Range % 94-102  
Recovery
The recovery of bovine Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-99  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
2000 0.243 0.252 0.248    
500 0.412 0.436 0.424    
120 0.793 0.754 0.774    
31.25 1.214 1.239 1.227    
7.81 1.795 1.724 1.760    
0 2.405 2.359 2.382    
References
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Function As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).
Subcellular Location Secreted, extracellular space
Protein Families Peptidase S1 family
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

KEGG: bta:280692

STRING: 9913.ENSBTAP00000008335

UniGene: Bt.12553

Q&A and Customer Reviews

Q&A
Q:

a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?

A:
Thanks for your inquiry.
The antibody used in the kit is rabbit polyclonal antibody.
The standard is purified crude extract of native samples.
Customer Reviews

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