Bovine Interleukin 2,IL-2 ELISA Kit

Instructions
Code CSB-E09810b
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 2
Alternative Names IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
Abbreviation IL2
Uniprot No. P05016
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %81-90
1:2Average %100
Range %97-103
1:4Average %101
Range %98-105
1:8Average %94
Range %86-98
Recovery
The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9692-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.151 2.099 2.125 2.040
10001.683 1.656 1.670 1.585
5001.284 1.270 1.277 1.192
2500.846 0.833 0.840 0.755
1250.502 0.498 0.500 0.415
62.50.367 0.356 0.362 0.277
31.250.221 0.213 0.217 0.132
00.086 0.084 0.085  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Gene References into Functions
  1. Pretreatment of neonatal PBMC with IL-1beta, TNF-alpha or IFN-gamma promotes mitogenic response to ConA through up-regulating the production of IL-2 and the expression of the mature IL-2 receptor. PMID: 12906107
Subcellular Location Secreted
Protein Families IL-2 family
Database Links

KEGG: bta:280822

STRING: 9913.ENSBTAP00000027819

UniGene: Bt.341

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