Bovine Interleukin 2,IL-2 ELISA Kit

Code CSB-E09810b
Size 96T,5×96T,10×96T
Price Request a Quote
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
Interested in a trial size? Please leave a message below.
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name
interleukin 2
Alternative Names
IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
Abbreviation
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %81-90
1:2Average %100
Range %97-103
1:4Average %101
Range %98-105
1:8Average %94
Range %86-98
Recovery
The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9692-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.151 2.099 2.125 2.040
10001.683 1.656 1.670 1.585
5001.284 1.270 1.277 1.192
2500.846 0.833 0.840 0.755
1250.502 0.498 0.500 0.415
62.50.367 0.356 0.362 0.277
31.250.221 0.213 0.217 0.132
00.086 0.084 0.085  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Interleukin 2 (IL-2) is a cytokine that plays a central role in immune system regulation and T cell activation. Originally discovered as a T cell growth factor, IL-2 is primarily produced by activated CD4+ T helper cells and serves as a key mediator of adaptive immune responses. This cytokine promotes T cell proliferation, differentiation, and survival, while also influencing the development and function of regulatory T cells. IL-2 signaling occurs through the IL-2 receptor complex and activates multiple downstream pathways that maintain immune homeostasis and mount effective immune responses against pathogens.

The Bovine Interleukin 2, IL-2 ELISA Kit (CSB-E09810b) is designed for quantitative measurement of IL-2 in Bos taurus samples. This sandwich-based assay accommodates serum, plasma, and tissue homogenates with a detection range of 31.25 pg/mL to 2000 pg/mL and sensitivity of 7.8 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength. This provides researchers with a reliable tool for studying bovine IL-2 levels in various biological samples.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This bovine interleukin 2 ELISA kit has been used in research focused on immune function and inflammatory response monitoring in cattle. The kit allows quantitative measurement of interleukin 2 concentrations in bovine biological samples as part of comprehensive cytokine profiling studies.

Immune function assessment - Measurement of interleukin 2 alongside other cytokines and immunoglobulins to evaluate overall immune status in bovine subjects
Inflammatory biomarker profiling - Quantification of interleukin 2 as part of multi-analyte panels including various interleukins and tumor necrosis factor to assess inflammatory responses
Serum-based cytokine analysis - Detection of interleukin 2 concentrations in bovine serum samples for immunological research applications

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Gene References into Functions
  1. Pretreatment of neonatal PBMC with IL-1beta, TNF-alpha or IFN-gamma promotes mitogenic response to ConA through up-regulating the production of IL-2 and the expression of the mature IL-2 receptor. PMID: 12906107
Subcellular Location
Secreted.
Protein Families
IL-2 family
Database Links

KEGG: bta:280822

STRING: 9913.ENSBTAP00000027819

UniGene: Bt.341

CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2025 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1