Code | CSB-E09810b |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 85 | ||||
Range % | 81-90 | |||||
1:2 | Average % | 100 | ||||
Range % | 97-103 | |||||
1:4 | Average % | 101 | ||||
Range % | 98-105 | |||||
1:8 | Average % | 94 | ||||
Range % | 86-98 |
The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 89-97 | ||||
EDTA plasma (n=4) | 96 | 92-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Interleukin 2 (IL-2) is a cytokine that plays a central role in immune system regulation and T cell activation. Originally discovered as a T cell growth factor, IL-2 is primarily produced by activated CD4+ T helper cells and serves as a key mediator of adaptive immune responses. This cytokine promotes T cell proliferation, differentiation, and survival, while also influencing the development and function of regulatory T cells. IL-2 signaling occurs through the IL-2 receptor complex and activates multiple downstream pathways that maintain immune homeostasis and mount effective immune responses against pathogens.
The Bovine Interleukin 2, IL-2 ELISA Kit (CSB-E09810b) is designed for quantitative measurement of IL-2 in Bos taurus samples. This sandwich-based assay accommodates serum, plasma, and tissue homogenates with a detection range of 31.25 pg/mL to 2000 pg/mL and sensitivity of 7.8 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength. This provides researchers with a reliable tool for studying bovine IL-2 levels in various biological samples.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This bovine interleukin 2 ELISA kit has been used in research focused on immune function and inflammatory response monitoring in cattle. The kit allows quantitative measurement of interleukin 2 concentrations in bovine biological samples as part of comprehensive cytokine profiling studies.
• Immune function assessment - Measurement of interleukin 2 alongside other cytokines and immunoglobulins to evaluate overall immune status in bovine subjects
• Inflammatory biomarker profiling - Quantification of interleukin 2 as part of multi-analyte panels including various interleukins and tumor necrosis factor to assess inflammatory responses
• Serum-based cytokine analysis - Detection of interleukin 2 concentrations in bovine serum samples for immunological research applications
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