Bovine Interleukin 2,IL-2 ELISA Kit

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Bovine Interleukin 2,IL-2 ELISA Kit

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Protocol

Code	CSB-E09810b-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
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Customer Reviews and Q&A
Target Background

Target Name

interleukin 2

Alternative Names

IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit

Abbreviation

IL2

Uniprot No.

P05016

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156ng/mL-10ng/mL

Sensitivity

0.078ng/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	99
	Range %	92-106
1:4	Average %	94
	Range %	89-99
1:8	Average %	101
	Range %	97-105

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
10	3.011	2.934	2.972	2.849
5	1.914	1.967	1.940	1.817
2.5	1.050	1.028	1.039	0.916
1.25	0.615	0.609	0.612	0.489
0.625	0.288	0.301	0.295	0.171
0.312	0.181	0.191	0.186	0.063
0.156	0.146	0.154	0.150	0.027
0	0.121	0.125	0.123

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Gene References into Functions

Pretreatment of neonatal PBMC with IL-1beta, TNF-alpha or IFN-gamma promotes mitogenic response to ConA through up-regulating the production of IL-2 and the expression of the mature IL-2 receptor. PMID: 12906107

Subcellular Location

Secreted.

Protein Families

IL-2 family

Database Links

KEGG: bta:280822

STRING: 9913.ENSBTAP00000027819

UniGene: Bt.341

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Related pathways

Jak-STAT signaling pathway PI3K-Akt signaling pathway T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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