Bovine Interleukin 2,IL-2 ELISA Kit

This ELISA kit provides quantitative measurement of interleukin-2 (IL-2) in bovine samples, supporting immunological research where T-cell activation and proliferative signaling are central to the study design. Because bovine IL-2 is a critical readout in vaccination trials, parasite challenge models, and mastitis immunology, this kit is engineered to capture the cytokine across both resting and stimulated conditions.

Detection Range: The 0.156–10 ng/mL range spans from baseline circulating IL-2 levels through the elevated concentrations seen in mitogen-stimulated PBMC supernatants and inflamed tissue homogenates. Most activated bovine lymphocyte cultures produce IL-2 within this window, minimizing the need for sample dilution or concentration steps.

Sensitivity: At 0.078 ng/mL, the kit detects IL-2 in unstimulated or weakly stimulated samples where secretion is minimal, such as early post-vaccination time points or naïve control conditions that still require quantifiable baseline values.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables paired systemic and local immune profiling — for example, comparing circulating IL-2 with mammary gland or lymph node tissue levels during infectious challenge studies.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate limited-volume calf blood draws and high-throughput screening of multiple vaccination cohorts within a single working day.

This kit is directly applicable to bovine vaccine efficacy studies, Th1 immune response characterization, and infectious disease models including bovine tuberculosis and theileriosis.