Bovine Pregnancy Specific Protein B(PSPB)ELISA Kit

Instructions
Code CSB-E13353B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Pregnancy-associated glycoprotein 1
Alternative Names Pregnancy-associated glycoprotein 1 ELISA Kit; PAG 1 ELISA Kit; EC 3.4.23.- ELISA Kit; Pregnancy-specific protein B ELISA Kit; PSP-B ELISA Kit
Abbreviation PAG1
Uniprot No. Q29432
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.06 ng/mL-24 ng/mL
Sensitivity 0.06 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine PSPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-104  
1:2 Average % 94  
Range % 88-105  
1:4 Average % 90  
Range % 85-96  
1:8 Average % 97  
Range % 89-105  
Recovery
The recovery of bovine PSPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 84-94  
EDTA plasma (n=4) 97 90-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
24 0.155 0.146 0.151    
4.5 0.344 0.332 0.338    
0.75 0.571 0.582 0.577    
0.21 1.006 0.998 1.002    
0.06 1.562 1.575 1.569    
0 2.191 2.223 2.207    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Appears to be proteolytically inactive.
Gene References into Functions
  1. This study examines expression patterns of the genes SERPINA14, PAG, and PAG2 at the fetal-maternal interface in dairy heifers experimentally infected with N caninum during the second trimester of pregnancy, when most abortions takes place in natural conditions. PMID: 27045629
  2. The clinical implications of these findings are that following infection with N. caninum, most cows will experience some level of placental damage and that this injury correlates with foetal fluid PAG levels and pH. PMID: 26936628
  3. These findings indicate that IFN-gamma production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma pregnancy-associated glycoproteins levels. PMID: 24456132
  4. Restraint stress for 2 h during early pregnancy in heifers increased blood cortisol, but it did not affect the concentrations of progesterone and PAG-1 between days 30 to 40 of gestation. PMID: 21872319
Subcellular Location Secreted, extracellular space
Protein Families Peptidase A1 family
Tissue Specificity Trophoblast and placental tissue. Produced specifically in the invasive binucleate cells of the placenta. Becomes detectable in maternal serum soon after implantation.
Database Links

KEGG: bta:281964

STRING: 9913.ENSBTAP00000025593

UniGene: Bt.15401

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