Bovine Thrombin-antithrombin III Complex, TAT-III ELISA Kit

Code CSB-E14133B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
Thrombin-antithrombin III Complex, TAT-III
Bos taurus (Bovine)
Sample Types
serum, plasma
Detection Range
1.56 pg/mL-100 pg/mL
0.78 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
To assess the linearity of the assay, samples were spiked with high concentrations of bovine TAT-III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:20 Average % 92    
Range % 88-96    
1:40 Average % 85    
Range % 80-90    
1:80 Average % 104    
Range % 100-110    
1:160 Average % 97    
Range % 92-101    
The recovery of bovine TAT-III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
Sample Type Average % Recovery Range    
Serum (n=5) 95 90-100    
EDTA plasma (n=4) 89 85-93    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
pg/ml OD1 OD2 Average      
100 0.159 0.163 0.161      
50 0.279 0.267 0.273      
25 0.455 0.471 0.463      
12.5 0.724 0.704 0.714      
6.25 1.071 1.006 1.039      
3.12 1.279 1.322 1.301      
1.56 1.683 1.718 1.701      
0 2.106 2.235 2.171      
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Bovine TAT-III ELISA Kit was designed for the quantitative measurement of Bovine TAT-III protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.78 pg/mL.

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