Bovine Transthyretin(TTR) ELISA kit

Code CSB-EL025270BO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
transthyretin
Alternative Names
TTR ELISA Kit; Transthyretin ELISA Kit; Prealbumin ELISA Kit
Abbreviation
TTR
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
312.5 ng/mL-5000 ng/mL
Sensitivity
312.5 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 95  
Range % 91-97  
1:200 Average % 103  
Range % 99-107  
1:400 Average % 98  
Range % 93-103  
1:800 Average % 92  
Range % 87-95  
Recovery
The recovery of bovine TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 94-104  
EDTA plasma (n=4) 89 84-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
5000 0.147 0.153 0.150    
2500 0.266 0.275 0.271    
1250 0.511 0.512 0.512    
625 0.905 0.903 0.904    
312.5 1.674 1.787 1.731    
0 2.689 2.599 2.644    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine TTR ELISA Kit was designed for the quantitative measurement of Bovine TTR protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 312.5 ng/mL-5000 ng/mL and the sensitivity is 312.5 ng/mL.

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Target Background

Function
(From Uniprot)
Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Subcellular Location
Secreted.
Protein Families
Transthyretin family
Tissue Specificity
Detected in serum (at protein level).
Database Links

KEGG: bta:280948

STRING: 9913.ENSBTAP00000014585

UniGene: Bt.207

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