Bovine Transthyretin(TTR) ELISA kit

Code CSB-EL025270BO
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Bovine TTR ELISA Kit was designed for the quantitative measurement of Bovine TTR protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 312.5 ng/mL-5000 ng/mL and the sensitivity is 312.5 ng/mL.

Target Name transthyretin
Alternative Names TTR ELISA Kit; Transthyretin ELISA Kit; Prealbumin ELISA Kit
Abbreviation TTR
Uniprot No. O46375
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 312.5 ng/mL-5000 ng/mL
Sensitivity 312.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 95  
Range % 91-97  
1:200 Average % 103  
Range % 99-107  
1:400 Average % 98  
Range % 93-103  
1:800 Average % 92  
Range % 87-95  
Recovery
The recovery of bovine TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 94-104  
EDTA plasma (n=4) 89 84-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
5000 0.147 0.153 0.150    
2500 0.266 0.275 0.271    
1250 0.511 0.512 0.512    
625 0.905 0.903 0.904    
312.5 1.674 1.787 1.731    
0 2.689 2.599 2.644    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Subcellular Location Secreted.
Protein Families Transthyretin family
Tissue Specificity Detected in serum (at protein level).
Database Links

KEGG: bta:280948

STRING: 9913.ENSBTAP00000014585

UniGene: Bt.207

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