Bovine luteinizing hormone (LH) ELISA kit

Code CSB-E12826B
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Alternative Names
LHBLutropin subunit beta ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit; Lutropin beta chain ELISA kit
Abbreviation
LH
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.25 mIU/mL-100 mIU/mL
Sensitivity
1.56 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 99  
Range % 95-102  
1:2 Average % 87  
Range % 84-90  
1:4 Average % 88  
Range % 83-94  
1:8 Average % 100  
Range % 95-105  
Recovery
The recovery of bovine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-95  
EDTA plasma (n=4) 87 85-92  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average    
100 0.361 0.368 0.365    
37.5 0.538 0.567 0.553    
18.75 0.781 0.765 0.773    
6.25 1.156 1.126 1.141    
1.25 1.662 1.698 1.680    
0 2.161 2.178 2.170    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine luteinizing hormone (LH) ELISA Kit was designed for the quantitative measurement of Bovine luteinizing hormone (LH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.25 mIU/mL-100 mIU/mL and the sensitivity is 1.56 mIU/mL.

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Target Background

Function
(From Uniprot)
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit beta family
Database Links
CUSABIO guaranteed quality
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