Bovine luteinizing hormone(LH)ELISA kit

Instructions
Code CSB-E12826B
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name luteinizing hormone(LH)ELISA kit
Alternative Names LHBLutropin subunit beta ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit; Lutropin beta chain ELISA kit
Abbreviation LH
Uniprot No. P04651
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 mIU/mL-100 mIU/mL
Sensitivity 1.56 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 99  
Range % 95-102  
1:2 Average % 87  
Range % 84-90  
1:4 Average % 88  
Range % 83-94  
1:8 Average % 100  
Range % 95-105  
Recovery
The recovery of bovine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-95  
EDTA plasma (n=4) 87 85-92  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average    
100 0.361 0.368 0.365    
37.5 0.538 0.567 0.553    
18.75 0.781 0.765 0.773    
6.25 1.156 1.126 1.141    
1.25 1.662 1.698 1.680    
0 2.161 2.178 2.170    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: bta:280839

STRING: 9913.ENSBTAP00000048635

UniGene: Bt.12926

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