Bovine luteinizing hormone (LH) ELISA kit

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Bovine luteinizing hormone (LH) ELISA kit

Citations (7) Protocol

Code	CSB-E12826B
Size	96T,5×96T,10×96T
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Citations (7)
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Description

This Bovine luteinizing hormone (LH) ELISA Kit was designed for the quantitative measurement of Bovine luteinizing hormone (LH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.25 mIU/mL-100 mIU/mL and the sensitivity is 1.56 mIU/mL.

Alternative Names

LHBLutropin subunit beta ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit; Lutropin beta chain ELISA kit

Abbreviation

Uniprot No.

P04651

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.25 mIU/mL-100 mIU/mL

Sensitivity

1.56 mIU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	95-102
1:2	Average %	87
	Range %	84-90
1:4	Average %	88
	Range %	83-94
1:8	Average %	100
	Range %	95-105

Recovery

The recovery of bovine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-95
EDTA plasma (n=4)	87	85-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average
100	0.361	0.368	0.365
37.5	0.538	0.567	0.553
18.75	0.781	0.765	0.773
6.25	1.156	1.126	1.141
1.25	1.662	1.698	1.680
0	2.161	2.178	2.170

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum Y Wang,researchsquare,2022
Epitope-based in silico peptide design yields peptide-directed antibodies that recognize the buffalo luteinizing hormone V Nayan,International Journal of Biological Macromolecules,2021
A study on stress response and fertility parameters in phenotypically thermotolerant and thermosensitive dairy cows during summer heat stress I Nanas,Reprod Domest Anim,2020
Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones Umer S, et al,Genes,2019
Association among lipopolysaccharide, the transforming growth factor-beta superfamily, follicular growth, and transcription factors in spontaneous bovine ovarian cysts Colakoglu H E, et al,Domestic Animal Endocrinology,2019
Urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed-artificial insemination in murrah buffalo (Bubalus bubalis) RM Selvam..et al,REPRODUCTION IN DOMESTIC ANIMALS,2017
Exploration of Luteinizing hormone in murrah buffalo (Bubalus bubalis) urine: Extended surge window opens door for estrus prediction Ramu Muthu Selvam.et al,General and Comparative Endocrinology,2016

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Function (From Uniprot)	Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Subcellular Location	Secreted.
Protein Families	Glycoprotein hormones subunit beta family
Database Links	KEGG: bta:280839 STRING: 9913.ENSBTAP00000048635 UniGene: Bt.12926

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St Ste 610-322 Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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