Bovine pulmonary surfactant-associated protein B,SP-B ELISA Kit

Code CSB-E13834B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
surfactant protein B
Alternative Names
SFTPB ELISA Kit; SFTP3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; 6 kDa protein ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
Uniprot No.
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
9.37 ng/mL-600 ng/mL
2.34 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %97
Range %92-103
1:2Average %91
Range %85-97
1:4Average %90
Range %88-94
1:8Average %100
Range %96-105
The recovery of bovine SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-93
EDTA plasma (n=4)106103-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
6002.238 2.412 2.325 2.191
3001.603 1.620 1.612 1.478
1501.054 1.102 1.078 0.944
750.766 0.771 0.769 0.635
37.50.534 0.580 0.557 0.423
18.750.322 0.329 0.326 0.192
9.370.234 0.219 0.227 0.093
00.132 0.135 0.134
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Bovine SFTPB ELISA Kit was designed for the quantitative measurement of Bovine SFTPB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.37 ng/mL-600 ng/mL and the sensitivity is 2.34 ng/mL.

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Target Background

(From Uniprot)
Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Gene References into Functions
  1. Measurements of the depth of SP-B(CTERM) indicated the peptide center positions ~8A more deeply than the phosphate headgroups, a topology that may allow the peptide to promote functional lipid structures without causing micellization upon compression. PMID: 22252270
  2. results suggest the hydrophobic surfactant protein, SP-B, protects alveolar surfactant phospholiopids from hydrolysis mediated by multiple sPLA(2) in both vesicles (alveolar subphase) and monomolecular films (air-liquid interface) PMID: 22037357
  3. The hydrophobic surfactant proteins SP-B and SP-C induce formation of bicontinuous inverse cubic phases. PMID: 20409474
  4. Reactive oxygen species inactivation of lipid extract surfactant B (SP-B) biophysical activity appears to involve modification of a maximum of three amino acids: Trp9, Met29, and Met65. PMID: 17425286
Subcellular Location
Secreted, extracellular space, surface film.
Database Links
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