Bovine pulmonary surfactant-associated protein C,SP-C ELISA Kit

Instructions
Code CSB-E13835B
Size 96T,5×96T,10×96T
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Product Details

Target Name surfactant protein C
Alternative Names SFTPC ELISA Kit; SFTP2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit
Abbreviation SFTPC
Uniprot No. P15783
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %96
Range %93-99
1:2Average %110
Range %107-113
1:4Average %95
Range %91-99
1:8Average %90
Range %87-93
Recovery
The recovery of bovine SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-96
EDTA plasma (n=4)106101-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
8002.197 2.168 2.183 2.028
4001.705 1.732 1.719 1.564
2001.192 1.168 1.180 1.025
1000.658 0.689 0.674 0.519
500.451 0.468 0.460 0.305
250.367 0.368 0.368 0.213
12.50.268 0.251 0.260 0.105
00.154 0.156 0.155
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Gene References into Functions
  1. The hydrophobic surfactant proteins SP-B and SP-C induce formation of bicontinuous inverse cubic phases. PMID: 20409474
Subcellular Location Secreted, extracellular space, surface film
Database Links

KEGG: bta:282071

STRING: 9913.ENSBTAP00000016672

UniGene: Bt.8126

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