Canine C-Reactive Protein,CRP ELISA Kit

Code CSB-E14262c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name C-Reactive Protein,CRP
Abbreviation CRP
Uniprot No. T2KEN6
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.312 μg/mL-20 μg/mL
Sensitivity 0.078 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of canine CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %98
Range %94-103
1:2Average %89
Range %84-96
1:4Average %97
Range %90-108
1:8Average %89
Range %84-95
The recovery of canine CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9587-99
EDTA plasma (n=4)9287-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
203.105 3.004 3.055 2.953
102.654 2.667 2.661 2.559
51.778 1.786 1.782 1.680
2.51.026 0.989 1.008 0.906
1.250.468 0.478 0.473 0.371
0.6250.261 0.281 0.271 0.169
0.3120.158 0.167 0.163 0.061
00.103 0.101 0.102  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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