Canine Interferon alpha-1/2 ELISA kit

Code CSB-E09849c
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Interferon alpha-1/2
Alternative Names
Interferon alpha-1/2 ELISA Kit; IFN-alpha-1/2 ELISA Kit
Abbreviation
IFN-A1/2
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
3.12 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine IFN-α1/2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %86-99
1:2Average %100
Range %94-109
1:4Average %92
Range %89-96
1:8Average %87
Range %83-96
Recovery
The recovery of canine IFN-α1/2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-96
EDTA plasma (n=4)9488-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
8002.815 2.824 2.820 2.726
4002.417 2.425 2.421 2.327
2001.834 1.863 1.849 1.755
1001.042 1.112 1.077 0.983
500.518 0.563 0.541 0.447
250.248 0.269 0.259 0.165
12.50.126 0.131 0.129 0.035
00.091 0.096 0.094  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog IFN-A1/2 ELISA Kit was designed for the quantitative measurement of Dog IFN-A1/2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

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Target Background

Function
(From Uniprot)
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Subcellular Location
Secreted.
Protein Families
Alpha/beta interferon family
Database Links

KEGG: cfa:100685213

UniGene: Cfa.4550

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