Canine Relaxin(RLN) ELISA Kit

Code CSB-E14982c
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Relaxin(RLN)
Alternative Names
RLN ELISA Kit; Prorelaxin [Cleaved into: Relaxin B chain; Relaxin A chain] ELISA Kit
Abbreviation
RLN
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine RLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 84-90
1:2 Average % 95
Range % 91-99
1:4 Average % 88
Range % 84-93
1:8 Average % 97
Range % 93-100
Recovery
The recovery of canine RLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 101-107
EDTA plasma (n=4) 99 94-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 2.366 2.504 2.435 2.269
100 1.644 1.602 1.623 1.457
50 0.901 0.964 0.933 0.767
25 0.487 0.468 0.478 0.312
12.5 0.319 0.327 0.323 0.157
6.25 0.261 0.268 0.265 0.099
3.12 0.206 0.196 0.201 0.035
0 0.161 0.171 0.166  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog RLN ELISA Kit was designed for the quantitative measurement of Dog RLN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

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Target Background

Function
(From Uniprot)
Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.
Gene References into Functions
  1. the luteal expression and localization of the RLN system was investigated by immunohistochemistry using custom-made antibodies and semi-quantitative PCR, at selected time points during gestation PMID: 29364921
  2. The blood levels of multiple steroids and other hormones in adult male dogs with or without prostatic hyperplasia are reported. PMID: 23279510
  3. The expression of relaxin and its receptors in canine mammary neoplasm tissue were studied, indicating a possible significant role of relaxin as an inducer of connective tissue remodelling. PMID: 19754574
Subcellular Location
Secreted.
Protein Families
Insulin family
Tissue Specificity
Placenta; syncytiotrophoblast.
Database Links
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