Target Name
Retinol binding protein 4(RBP-4)
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Detection Range
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Sensitivity
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Detection Wavelength
450 nm
Research Area
Signal Transduction
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of canine RBP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
86-96 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-99 |
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| 1:8 |
Average % |
92 |
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| Range % |
85-97 |
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Recovery
| The recovery of canine RBP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-96 |
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| EDTA plasma (n=4) |
97 |
95-100 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| μg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 80 |
1.944 |
2.111 |
2.028 |
1.867 |
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| 40 |
1.326 |
1.240 |
1.283 |
1.122 |
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| 20 |
0.777 |
0.787 |
0.782 |
0.621 |
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| 10 |
0.445 |
0.430 |
0.438 |
0.277 |
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| 5 |
0.299 |
0.286 |
0.293 |
0.132 |
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| 2.5 |
0.231 |
0.242 |
0.237 |
0.076 |
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| 1.25 |
0.201 |
0.206 |
0.204 |
0.043 |
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| 0 |
0.158 |
0.164 |
0.161 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
