Canine brain natriuretic peptide(BNP)ELISA Kit

Code CSB-E17436c
Size 96T,5×96T,10×96T
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Product Details

Target Name
brain natriuretic peptide(BNP)
Alternative Names
NPPBNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 34 ELISA kit; BNP-34); Brain natriuretic peptide 29 ELISA kit; BNP-29)] ELISA kit
Abbreviation
BNP
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
3.12 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-105
1:2Average %91
Range %88-96
1:4Average %95
Range %90-102
1:8Average %97
Range %92-103
Recovery
The recovery of canine BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9893-105
EDTA plasma (n=4)9692-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
8001.867 1.953 1.910 1.706
4001.524 1.600 1.562 1.358
2001.166 1.124 1.145 0.941
1000.812 0.862 0.837 0.633
500.524 0.563 0.544 0.340
250.375 0.401 0.388 0.184
12.50.278 0.259 0.269 0.065
00.203 0.204 0.204  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Dog BNP ELISA Kit was designed for the quantitative measurement of Dog BNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

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Target Background

Function
(From Uniprot)
Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.; May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.
Subcellular Location
Secreted.; [Brain natriuretic peptide 32]: Secreted.
Protein Families
Natriuretic peptide family
Tissue Specificity
Brain and also in atria, but at much lower levels than ANP.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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