Canine cardiac isoform of Tropnin T,cTnT ELISA Kit

Instructions
Code CSB-E14377c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cardiac isoform of Tropnin T,cTnT
Abbreviation cTnT
Uniprot No. J9P6P7
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine cTnT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 85-105  
1:2 Average % 95  
Range % 85-103  
1:4 Average % 94  
Range % 82-100  
1:8 Average % 95  
Range % 85-105  
Recovery
The recovery of canine cTnT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 82-104  
EDTA plasma (n=4) 92 85-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.215 2.313 2.264 2.123  
500 1.698 1.706 1.702 1.561  
250 1.113 1.131 1.122 0.981  
125 0.627 0.650 0.639 0.498  
62.5 0.382 0.403 0.393 0.252  
31.2 0.266 0.282 0.274 0.133  
15.6 0.202 0.198 0.200 0.059  
0 0.144 0.138 0.141    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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