Chicken Heat Shock Protein 90,HSP-90 ELISA Kit

Code CSB-E12873C
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Alternative Names HSP90AA1 ELISA Kit; HSPCAHeat shock protein HSP 90-alpha ELISA Kit
Abbreviation HSP90AA1
Uniprot No. P11501
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of chicken HSP-90 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %95
Range %85-103
1:2Average %89
Range %85-96
1:4Average %93
Range %83-99
1:8Average %95
Range %89-98
The recovery of chicken HSP-90 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-101
EDTA plasma (n=4)9587-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
1002.428 2.388 2.408 2.303
501.994 1.935 1.965 1.860
251.362 1.334 1.348 1.243
12.50.830 0.868 0.849 0.744
6.250.618 0.585 0.602 0.497
3.120.345 0.342 0.344 0.239
1.560.251 0.262 0.257 0.152
00.103 0.107 0.105
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from Hsp90 which acquires an open conformation for the next cycle.
Subcellular Location Cytoplasm. Melanosome.
Protein Families Heat shock protein 90 family
Database Links

KEGG: gga:423463

STRING: 9031.ENSGALP00000018498

UniGene: Gga.6482


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