Chicken Interleukin 10,IL-10 ELISA Kit

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Chicken Interleukin 10,IL-10 ELISA Kit

Specification
Target

Code

CSB-E12835C

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

Q6A2H4

Lead Time

3-5 working days

Abbreviation

IL10

Target Name

interleukin 10

Alias

CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor

Species

Gallus gallus (Chicken)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1 pg/mL-200 pg/mL

Sensitivity

0.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Target Details

This protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

HGNC

5962

RGD

2886

MGI

96537

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-101
1:2	Average %	97
	Range %	94-105
1:4	Average %	95
	Range %	89-100
1:8	Average %	92
	Range %	87-96

Recovery

The recovery of chicken IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	97	93-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.155	0.152	0.154
50	0.334	0.348	0.341
16	0.515	0.502	0.509
4	0.969	0.975	0.972
1	1.436	1.463	1.450
0	2.658	2.667	2.663

References

Panax notoginseng saponins mitigate ovariectomy-induced bone loss and inhibitMarrow adiposity in rats. Fan JZ. et al,Menopause.,2015 Jun 1.
Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015 Oct;64(5):408-16.
Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015 Jul 16:1-35.
Effect of salinity changes on olfactory memory-related genes and hormones in adult chum salmon Oncorhynchus keta Kim NN et al,Comp Biochem Physiol A Mol Integr Physiol,2015 Sep;187:40-7.
The molecular characterization and immune protection of microneme 2 of Eimeria acervulina ZC Zhang. et al,Veterinary Parasitology,2015 215: 96-105.
The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,/
Effects of yeast cell wall on growth performance, immune responses and intestinal short chain fatty acid concentrations of broiler in an experimental necrotic enteritis model Guang-DaXue.et al,Animal Nutrition,Aug 18
Dextran sulphate sodium (DSS) causes intestinal histopathology and inflammatory changes consistent with increased gut leakiness in chickens Zou X.et al,Br Poult Sci,Dec 21
The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,June 25
Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers Shahid Ali Rajput, et al,Toxins,January 7
Xylanase and Fermented Polysaccharide of Hericium caputmedusae Reduce Pathogenic Infection of Broilers by Improving Antioxidant and Anti-Inflammatory Properties Sitong Zhang, et al,Oxidative Medicine and Cellular Longevity,December 30

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Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location	Secreted
Protein Families	IL-10 family
Tissue Specificity	Expressed predominantly in bursa of Fabricius and cecal tonsils with low levels in thymus, liver and lung.
Database Links	KEGG: gga:428264 STRING: 9031.ENSGALP00000001310 UniGene: Gga.46641
Pathway	Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway

IL10 Protein

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Code	Product Name	Source
CSB-YP715134CH CSB-EP715134CH CSB-BP715134CH CSB-MP715134CH	Recombinant Chicken Interleukin-10(IL10)	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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