Chicken Interleukin 10,IL-10 ELISA Kit

Code CSB-E12835C
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Chicken IL10 ELISA Kit was designed for the quantitative measurement of Chicken IL10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1 pg/mL-200 pg/mL and the sensitivity is 0.5 pg/mL.

Target Name interleukin 10
Alternative Names IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit
Abbreviation IL10
Uniprot No. Q6A2H4
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 1 pg/mL-200 pg/mL
Sensitivity 0.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 90-101  
1:2 Average % 97  
Range % 94-105  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 92  
Range % 87-96  
Recovery
The recovery of chicken IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 97 93-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
200 0.155 0.152 0.154    
50 0.334 0.348 0.341    
16 0.515 0.502 0.509    
4 0.969 0.975 0.972    
1 1.436 1.463 1.450    
0 2.658 2.667 2.663    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
Subcellular Location Secreted.
Protein Families IL-10 family
Tissue Specificity Expressed predominantly in bursa of Fabricius and cecal tonsils with low levels in thymus, liver and lung.
Database Links

KEGG: gga:428264

STRING: 9031.ENSGALP00000001310

UniGene: Gga.46641

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