Chicken Interleukin 10,IL-10 ELISA Kit

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Chicken Interleukin 10,IL-10 ELISA Kit

Name: Chicken Interleukin 10,IL-10 ELISA Kit
Brand: CUSABIO
SKU: CSB-E12835C

^Instructions

Code	CSB-E12835C
Size	96T,5×96T,10×96T
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

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Citations
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Target Data

Target Name

interleukin 10

Alternative Names

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit

Abbreviation

IL10

Uniprot No.

Q6A2H4

Species

Gallus gallus (Chicken)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1 pg/mL-200 pg/mL

Sensitivity

0.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-101
1:2	Average %	97
	Range %	94-105
1:4	Average %	95
	Range %	89-100
1:8	Average %	92
	Range %	87-96

Recovery

The recovery of chicken IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	97	93-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.155	0.152	0.154
50	0.334	0.348	0.341
16	0.515	0.502	0.509
4	0.969	0.975	0.972
1	1.436	1.463	1.450
0	2.658	2.667	2.663

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4 P Zhao,Veterinary Parasitology,2020
A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine Liu Y, et al,Journal of Immunology Research,2019
Egg White/Egg Shell based Biomimetic Hybrid Hydrogels for Bone Regeneration Keqing Huang, et al,Acs Biomaterials-Science & Engineering,2019
Effects of sodium butyrate on intestinal health and gut microbiota composition during intestinal inflammation progression in broilers Zou X,Poultry Science,2019
Oxidative Stress in Microbial Diseases: Pathogen, Host, and Therapeutics Novaes RD, et al,Oxidative Medicine And Cellular Longevity,2019
Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers Shahid Ali Rajput, et al,Toxins,2019
Xylanase and Fermented Polysaccharide of Hericium caputmedusae Reduce Pathogenic Infection of Broilers by Improving Antioxidant and Anti-Inflammatory Properties Sitong Zhang, et al,Oxidative Medicine and Cellular Longevity,2018
The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,2018
Dextran sulphate sodium (DSS) causes intestinal histopathology and inflammatory changes consistent with increased gut leakiness in chickens Zou X.et al,Br Poult Sci,2017
Effects of yeast cell wall on growth performance, immune responses and intestinal short chain fatty acid concentrations of broiler in an experimental necrotic enteritis model Guang-DaXue.et al,Animal Nutrition,2017
The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,2016
The molecular characterization and immune protection of microneme 2 of Eimeria acervulina ZC Zhang. et al,Veterinary Parasitology,2015
Effect of salinity changes on olfactory memory-related genes and hormones in adult chum salmon Oncorhynchus keta Kim NN et al,Comp Biochem Physiol A Mol Integr Physiol,2015
Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015
Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015
Panax notoginseng saponins mitigate ovariectomy-induced bone loss and inhibitMarrow adiposity in rats. Fan JZ. et al,Menopause.,2015

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Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location	Secreted
Protein Families	IL-10 family
Tissue Specificity	Expressed predominantly in bursa of Fabricius and cecal tonsils with low levels in thymus, liver and lung.
Database Links	KEGG: gga:428264 STRING: 9031.ENSGALP00000001310 UniGene: Gga.46641

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Related pathways

Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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