| Code |
CSB-E06755Ch |
| See More Details |
 |
| Product Type |
ELISA Kit |
| Size |
96T,5×96T,10×96T |
| Uniprot No. |
O73883
|
| Lead Time |
3-5 working days |
| Abbreviation |
IL-2 |
| Target Name |
Interleukin 2,IL-2 |
| Species |
Gallus gallus (Chicken) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.18 pg/mL-12 pg/mL |
| Sensitivity |
0.04 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Protocol |
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
89 |
|
| Range % |
85-96 |
|
| 1:2 |
Average % |
90 |
|
| Range % |
85-97 |
|
| 1:4 |
Average % |
99 |
|
| Range % |
94-106 |
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| 1:8 |
Average % |
97 |
|
| Range % |
92-104 |
|
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| Recovery |
| The recovery of chicken IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
100 |
92-109 |
|
| EDTA plasma (n=4) |
96 |
90-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 12 |
2.151 |
2.198 |
2.175 |
2.072 |
|
| 6 |
1.835 |
1.869 |
1.852 |
1.749 |
|
| 3 |
1.457 |
1.487 |
1.472 |
1.369 |
|
| 1.5 |
1.098 |
1.051 |
1.075 |
0.972 |
|
| 0.75 |
0.698 |
0.682 |
0.690 |
0.587 |
|
| 0.38 |
0.449 |
0.460 |
0.455 |
0.352 |
|
| 0.18 |
0.236 |
0.222 |
0.229 |
0.126 |
|
| 0 |
0.102 |
0.104 |
0.103 |
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| References |
-
Lactobacillus acidophilus as a live vehicle for oral immunization against chicken anemia virus Hassan Moeini et al,Tumor Biology,2010 90(1): 77-88
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Bursopentine as a Novel Immunoadjuvant Enhances both Humoral and Cell-Mediated Immune Responses to Inactivated H9N2 Avian Influenza Virus in Chickens /,clinical and vaccine immunology,2011 Sep;18(9):1497-502.
-
Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV /,Comparative Immunology, Microbiology and Infectious Diseases,May 34(3):227-36.
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Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV toMarek's Disease Virus (MDV) Type-1 VP22 protein Hassan Moeini et al,Virology Journal,2011 8:119
-
Omega-3 polyunsaturated fatty acids enrichment alters performance and immune response in infectious bursal disease challenged broilers Elham Maroufyan et al,Lipids in Health and Disease,2012 11: 15
-
Consumption of Oxidized Soybean Oil Increased
Intestinal Oxidative Stress and Affected Intestinal Immune Variables in
Yellow-feathered Broilers
Fangfang Liang. et al,Asian Australas. J. Anim. Sci.
,2015 Aug;28(8):1194-201.
-
Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015 Oct;64(5):408-16.
-
Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015 Jul 16:1-35.
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Immune responses to oral vaccination with Salmonella-delivered avian pathogenic Escherichia coli antigens and protective efficacy against colibacillosis. Lee JH.et al,Can J Vet Res.,2015 79(3):229-34.
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The molecular characterization and immune protection of microneme 2 of Eimeria acervulina ZC Zhang. et al,Veterinary Parasitology,2015 215: 96-105.
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Dietary Supplementation of Phoenix dactylifera Seeds Enhances Performance, Immune Response, and Antioxidant Status in Broilers Ali H. El-Far.et al,Oxidative Medicine and Cellular Longevity,/
-
Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,Virus Res.,220:129-35
-
The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,/
-
The effect of heat stress on intestinal integrity and Salmonella invasion in broiler birds Alhenaky A.et al,J Therm Biol.,Dec
-
Effects of modified montmorillonite adsorbent on performance, egg quality, serum biochemistry, oxidation status, and immune response of laying hens in late Qu, X. Y.et al,Livestock Science,Jan
-
The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,June 25
-
Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,Tropical Animal Science Journal,March 5
-
Effects of three probiotic Bacillus on growth performance, digestive enzyme activities, antioxidative capacity, serum immunity, and biochemical parameters in broilers Li Gong.et al,animal science journal,September 9
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Effects of Supplementing Laying Hens with Purified Amino Acid Prepared from Animal Blood N. Joshi, et al,Tropical animal science journal,April
-
The effects and combinational effects of Bacillus subtilis and montmorillonite supplementation on performance, egg quality, oxidation status, and immune response in laying hens J.F.Chen, et al,The Egyptian Journal of Neurology, Psychiatry and Neurosurgery,July 2
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Effect of the melatonin nuclear receptor RORα on monochromatic light-induced T-lymphocyte proliferation in chicken thymus Xiong J, et al,Immunology Letters,July 18
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