Chicken Interleukin 2,IL-2 ELISA Kit

Code CSB-E06755Ch
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Interleukin 2,IL-2
Abbreviation
IL-2
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.18 pg/mL-12 pg/mL
Sensitivity
0.04 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 85-96  
1:2 Average % 90  
Range % 85-97  
1:4 Average % 99  
Range % 94-106  
1:8 Average % 97  
Range % 92-104  
Recovery
The recovery of chicken IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 92-109  
EDTA plasma (n=4) 96 90-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
12 2.151 2.198 2.175 2.072  
6 1.835 1.869 1.852 1.749  
3 1.457 1.487 1.472 1.369  
1.5 1.098 1.051 1.075 0.972  
0.75 0.698 0.682 0.690 0.587  
0.38 0.449 0.460 0.455 0.352  
0.18 0.236 0.222 0.229 0.126  
0 0.102 0.104 0.103    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Chicken Interleukin 2 (IL-2) ELISA Kit is used for accurate and precise measurement of IL-2 in samples from Gallus gallus (Chicken) species. It is designed for use with serum, plasma, and tissue homogenates, and provides a detection range of 0.18 pg/mL-12 pg/mL, with a sensitivity of 0.04 pg/mL.

This ELISA Kit is based on the Sandwich assay principle and has a measurement wavelength of 450 nm. With an assay time of just 1-5 hours and a sample volume of 50-100 ul, this kit is both efficient and convenient for laboratory use.

This product is ideal for researchers in the field of Immunology, and has been cited in over 25 scientific publications. With its high sensitivity and accuracy, this kit will provide you with reliable results for your research needs.

Citations

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