Description |
This Chicken IL-2 ELISA Kit was designed for the quantitative measurement of Chicken IL-2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 pg/mL-12 pg/mL and the sensitivity is 0.04 pg/mL .
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Target Name |
Interleukin 2,IL-2 |
Abbreviation |
IL-2 |
Uniprot No. |
O73883 |
Species |
Gallus gallus (Chicken) |
Sample Types |
serum, plasma, tissue homogenates |
Detection Range |
0.18 pg/mL-12 pg/mL |
Sensitivity |
0.04 pg/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Immunology
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Assay Principle |
quantitative |
Measurement |
Sandwich |
Precision |
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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1:1 |
Average % |
89 |
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Range % |
85-96 |
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1:2 |
Average % |
90 |
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Range % |
85-97 |
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1:4 |
Average % |
99 |
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Range % |
94-106 |
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1:8 |
Average % |
97 |
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Range % |
92-104 |
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Recovery |
The recovery of chicken IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
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Serum (n=5) |
100 |
92-109 |
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EDTA plasma (n=4) |
96 |
90-104 |
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Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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12 |
2.151 |
2.198 |
2.175 |
2.072 |
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6 |
1.835 |
1.869 |
1.852 |
1.749 |
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3 |
1.457 |
1.487 |
1.472 |
1.369 |
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1.5 |
1.098 |
1.051 |
1.075 |
0.972 |
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0.75 |
0.698 |
0.682 |
0.690 |
0.587 |
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0.38 |
0.449 |
0.460 |
0.455 |
0.352 |
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0.18 |
0.236 |
0.222 |
0.229 |
0.126 |
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0 |
0.102 |
0.104 |
0.103 |
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ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
3-5 working days |