Chicken Interleukin 2,IL-2 ELISA Kit

Instructions
Code CSB-E06755Ch
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name Interleukin 2,IL-2
Abbreviation IL-2
Uniprot No. O73883
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.18 pg/mL-12 pg/mL
Sensitivity 0.04 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 85-96  
1:2 Average % 90  
Range % 85-97  
1:4 Average % 99  
Range % 94-106  
1:8 Average % 97  
Range % 92-104  
Recovery
The recovery of chicken IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 92-109  
EDTA plasma (n=4) 96 90-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
12 2.151 2.198 2.175 2.072  
6 1.835 1.869 1.852 1.749  
3 1.457 1.487 1.472 1.369  
1.5 1.098 1.051 1.075 0.972  
0.75 0.698 0.682 0.690 0.587  
0.38 0.449 0.460 0.455 0.352  
0.18 0.236 0.222 0.229 0.126  
0 0.102 0.104 0.103    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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