| Description |
This Chicken IL-2 ELISA Kit was designed for the quantitative measurement of Chicken IL-2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 pg/mL-12 pg/mL and the sensitivity is 0.04 pg/mL . |
| Target Name |
Interleukin 2,IL-2 |
| Abbreviation |
IL-2 |
| Uniprot No. |
O73883 |
| Species |
Gallus gallus (Chicken) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.18 pg/mL-12 pg/mL |
| Sensitivity |
0.04 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
89 |
|
| Range % |
85-96 |
|
| 1:2 |
Average % |
90 |
|
| Range % |
85-97 |
|
| 1:4 |
Average % |
99 |
|
| Range % |
94-106 |
|
| 1:8 |
Average % |
97 |
|
| Range % |
92-104 |
|
|
| Recovery |
| The recovery of chicken IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
100 |
92-109 |
|
| EDTA plasma (n=4) |
96 |
90-104 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 12 |
2.151 |
2.198 |
2.175 |
2.072 |
|
| 6 |
1.835 |
1.869 |
1.852 |
1.749 |
|
| 3 |
1.457 |
1.487 |
1.472 |
1.369 |
|
| 1.5 |
1.098 |
1.051 |
1.075 |
0.972 |
|
| 0.75 |
0.698 |
0.682 |
0.690 |
0.587 |
|
| 0.38 |
0.449 |
0.460 |
0.455 |
0.352 |
|
| 0.18 |
0.236 |
0.222 |
0.229 |
0.126 |
|
| 0 |
0.102 |
0.104 |
0.103 |
|
|
|
|
| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
