Chicken Interleukin 4,IL-4 ELISA Kit

Code CSB-E06756Ch
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name Interleukin 4,IL-4
Abbreviation IL-4
Uniprot No. Q5W4U1
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %89
Range %80-95
1:2Average %94
Range %88-101
1:4Average %93
Range %86-106
1:8Average %95
Range %82-100
The recovery of chicken IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-97
EDTA plasma (n=4)9285-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
8001.726 1.825 1.776 1.665
4001.336 1.383 1.360 1.249
2001.060 0.981 1.021 0.910
1000.714 0.706 0.710 0.599
500.481 0.475 0.478 0.367
250.307 0.292 0.300 0.189
12.50.227 0.214 0.221 0.110
00.108 0.113 0.111  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat


Most popular with customers


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 CUSABIO TECHNOLOGY LLC All Rights Reserved.