Chicken Parathyroid hormone,PTH ELISA Kit

Code CSB-E11880Ch
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
parathyroid hormone
Alternative Names
PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit
Abbreviation
PTH
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-100 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-95  
1:2 Average % 96  
Range % 92-103  
1:4 Average % 85  
Range % 81-86  
1:8 Average % 90  
Range % 83-98  
Recovery
The recovery of chicken PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 87-98  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
100 0.184 0.195 0.190    
50 0.325 0.350 0.338    
25 0.531 0.504 0.518    
12.5 1.006 1.026 1.016    
6.25 2.117 2.220 2.169    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Chicken PTH ELISA Kit was designed for the quantitative measurement of Chicken PTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 pg/mL-100 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion.
Gene References into Functions
  1. A single nucleotide polymorphism in parathyroid hormone affected the eggshell percentage and breaking strength, and it may be associated with the variation of serum calcium and PTH level. PMID: 20852100
Subcellular Location
Secreted.
Protein Families
Parathyroid hormone family
Database Links

KEGG: gga:396436

STRING: 9031.ENSGALP00000008579

UniGene: Gga.78

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