Chicken Parathyroid hormone,PTH ELISA Kit

Instructions
Code CSB-E11880Ch
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name parathyroid hormone
Alternative Names PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit
Abbreviation PTH
Uniprot No. P15743
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-100 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-95  
1:2 Average % 96  
Range % 92-103  
1:4 Average % 85  
Range % 81-86  
1:8 Average % 90  
Range % 83-98  
Recovery
The recovery of chicken PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 87-98  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
100 0.184 0.195 0.190    
50 0.325 0.350 0.338    
25 0.531 0.504 0.518    
12.5 1.006 1.026 1.016    
6.25 2.117 2.220 2.169    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion.
Gene References into Functions
  1. A single nucleotide polymorphism in parathyroid hormone affected the eggshell percentage and breaking strength, and it may be associated with the variation of serum calcium and PTH level. PMID: 20852100
Subcellular Location Secreted
Protein Families Parathyroid hormone family
Database Links

KEGG: gga:396436

STRING: 9031.ENSGALP00000008579

UniGene: Gga.78

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