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Chicken Parathyroid hormone,PTH ELISA Kit

Citations (7) Protocol
Code CSB-E11880Ch
Size 96T,5×96T,10×96T
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Product Details

Target Name
parathyroid hormone
Alternative Names
PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit
Abbreviation
PTH
Uniprot No.
P15743
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-100 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-95  
1:2 Average % 96  
Range % 92-103  
1:4 Average % 85  
Range % 81-86  
1:8 Average % 90  
Range % 83-98  
Recovery
The recovery of chicken PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 87-98  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
100 0.184 0.195 0.190    
50 0.325 0.350 0.338    
25 0.531 0.504 0.518    
12.5 1.006 1.026 1.016    
6.25 2.117 2.220 2.169    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Parathyroid hormone (PTH) is an endocrine regulator that maintains calcium homeostasis in vertebrates. The parathyroid glands secrete PTH to increase blood calcium levels by stimulating calcium reabsorption in the kidneys, promoting calcium absorption in the intestines, and mobilizing calcium from bone tissue. This hormone plays a key role in bone metabolism and mineral balance, making it a valuable biomarker for studying calcium-related disorders, bone diseases, and endocrine function in research applications.

The Chicken Parathyroid hormone PTH ELISA Kit (CSB-E11880Ch) provides quantitative measurement of parathyroid hormone in Gallus gallus samples. This competitive assay works with serum, plasma, and tissue homogenates. The detection range spans 6.25 pg/mL to 100 pg/mL with a sensitivity of 6.25 pg/mL. The assay requires 50-100 μL sample volume, operates with detection at 450 nm wavelength, and can be completed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in avian research to quantify parathyroid hormone levels in chicken plasma and serum samples. Applications include endocrine and metabolic studies examining calcium homeostasis and mineral metabolism in poultry research settings.

• Endocrine profiling - Measurement of parathyroid hormone as part of comprehensive hormone panels alongside vitamin D metabolites and thyroid hormones in chicken studies
• Mineral metabolism research - Measuring parathyroid hormone levels alongside serum calcium, phosphorus, magnesium, and albumin to study calcium homeostasis
• Nutritional studies - Evaluating parathyroid hormone responses in dietary intervention experiments that examine how different feed formulations affect poultry endocrine function
• Strain comparison studies - Analyzing parathyroid hormone variations between different chicken strains to understand genetic influences on calcium regulatory mechanisms

Citations

Related Products

PTH Proteins

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Target Background

Function
(From Uniprot)
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion.
Gene References into Functions
  1. A single nucleotide polymorphism in parathyroid hormone affected the eggshell percentage and breaking strength, and it may be associated with the variation of serum calcium and PTH level. PMID: 20852100
Subcellular Location
Secreted.
Protein Families
Parathyroid hormone family
Database Links

KEGG: gga:396436

STRING: 9031.ENSGALP00000008579

UniGene: Gga.78

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