Chicken growth hormone,GH ELISA KIT

Instructions
Code CSB-E09866Ch
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name growth hormone,GH
Alternative Names GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
Abbreviation GH
Uniprot No. P08998
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 625 pg/mL-10000 pg/mL
Sensitivity 625 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 86-98  
1:2 Average % 99  
Range % 92-105  
1:4 Average % 85  
Range % 80-89  
1:8 Average % 90  
Range % 83-98  
Recovery
The recovery of chicken GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 88-96  
EDTA plasma (n=4) 94 91-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
10000 0.154 0.145 0.150    
5000 0.261 0.244 0.253    
2500 0.538 0.534 0.536    
1250 1.113 1.139 1.126    
625 2.356 2.278 2.317    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Growth hormone plays an important role in growth control.
Gene References into Functions
  1. This is the first study to demonstrate that single nucleotide polymorphisms in GHSR can increase appetite, growth traits, expression and level of GHRL, suggesting a hunger signal role for endogenous GHRL. PMID: 27591070
  2. There is a negative glucocorticoid response-like element located in the +591/+609 region within the first intron of chicken growth hormone (cGH), which is essential for the down-regulation of cGH expression. PMID: 27834851
  3. It is shown that in all examined chicken populations the growth hormone gene is polymorphic. PMID: 26841491
  4. The molecular mechanisms underlying glucocorticoid recruitment of somatotrophs during embryogenesis were studied; these provide insight into glucocorticoid-induced developmental changes in other tissues and cell types. PMID: 25560830
  5. T185G growth hormone polymorphism was significantly associated with the egg production traits in chickens. PMID: 25062422
  6. GHRH stimulates testicular GH secretion in an autocrine or paracrine manner. PMID: 24508498
  7. The results indicate that high frequency of I, C and A alleles corresponds to the egg-laying chicken lines. PMID: 23285753
  8. A 1727-bp fragment of 5'-flanking region of chicken growth hormone (cGH) gene has been cloned and sequenced. PMID: 15229358
  9. small chicken GH expression and translation occurs in ocular tissues of chick embryos, in which its localization in the neural retina and the optic nerve head is distinct from that of the full-length protein PMID: 17008400
  10. The identification of 3 restriction enzyme polymorphic sites were detected in the growth hormone gene is reported. PMID: 17495076
  11. Results showed that there was no significant difference between dwarf and normal chickens in GH expression. PMID: 17681929
  12. results demonstrate presence of growth hormone and its receptors in the ovarian stroma with cortical follicles & in the wall of small and large follicles of the ovary during sexual maturation PMID: 18335240
  13. Positive somatotroph responses to corticosterone (CORT) are lost during chicken embryonic development and that glucocorticoid receptors are the primary regulator of CORT-induced growth hormone secretion. PMID: 19157766

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Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

KEGG: gga:378781

STRING: 9031.ENSGALP00000000327

UniGene: Gga.48744

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