Deer Insulin-like growth factors 1,IGF-1 ELISA Kit

Code CSB-E12644De
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Insulin-like growth factors 1,IGF-1
Abbreviation
IGF-1
Uniprot No.
Species
Deer
Sample Types
serum, plasma, tissue homogenates
Detection Range
1 ng/mL-200 ng/mL
Sensitivity
0.5 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of deer IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %98
Range %92-104
1:2Average %95
Range %89-104
1:4Average %99
Range %87-107
1:8Average %96
Range %90-102
Recovery
The recovery of deer IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9690-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
2000.175 0.174 0.175
500.333 0.316 0.325
160.622 0.640 0.631
41.040 1.048 1.044
11.416 1.389 1.403
02.577 2.633 2.605
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Deer IGF-1 ELISA Kit was designed for the quantitative measurement of Deer IGF-1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1 ng/mL-200 ng/mL and the sensitivity is 0.5 ng/mL .

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