Deer Insulin-like growth factors 1,IGF-1 ELISA Kit

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Deer Insulin-like growth factors 1,IGF-1 ELISA Kit

Citations (4) Protocol

Code	CSB-E12644De
Size	96T,5×96T,10×96T
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Product Details
Citations (4)
Customer Reviews and Q&A

Target Name

Insulin-like growth factors 1,IGF-1

Abbreviation

IGF-1

Uniprot No.

H9BDM1

Species

Deer

Sample Types

serum, plasma, tissue homogenates

Detection Range

1 ng/mL-200 ng/mL

Sensitivity

0.5 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of deer IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	95
	Range %	89-104
1:4	Average %	99
	Range %	87-107
1:8	Average %	96
	Range %	90-102

Recovery

The recovery of deer IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
200	0.175	0.174	0.175
50	0.333	0.316	0.325
16	0.622	0.640	0.631
4	1.040	1.048	1.044
1	1.416	1.389	1.403
0	2.577	2.633	2.605

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Deer IGF-1 ELISA Kit was designed for the quantitative measurement of Deer IGF-1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1 ng/mL-200 ng/mL and the sensitivity is 0.5 ng/mL .

Optimizing protein yield from growing deer antlers N Alegría-Aravena, MP López-Garrido,Frontiers in Veterinary Science,2025
SWATH-MS Quantitative Proteomic Analysis of Deer Antler from Two Regenerating and Mineralizing Sections M López-Pedrouso,Biology,2021
Reproductive responses to sexually active buck of does treated with melatonin when body weight/body condition is increasing orDecreasing Gallego-Calvo, L.et al, Animal Reproduction Science,2018
Detection and quantitation of insulin-like growth factor-1 in velvet antler by an enzyme-linked immunosorbent assay /,Consumer Electronics, Communications and Networks (CECNet),2011

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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