Dog Hepcidin(HAMP) ELISA kit

Code CSB-EL010124DO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
hepcidin antimicrobial peptide
Alternative Names
HAMP ELISA Kit; Hepcidin ELISA Kit
Abbreviation
HAMP
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 107
Range % 96-111
1:2 Average % 100
Range % 90-104
1:4 Average % 97
Range % 90-101
1:8 Average % 91
Range % 82-95
Recovery
The recovery of dog HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 84-95
EDTA plasma (n=4) 107 99-111
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 1.971 1.912 1.942 1.810
100 1.482 1.477 1.480 1.348
50 0.976 0.988 0.982 0.850
25 0.546 0.556 0.551 0.419
12.5 0.402 0.378 0.390 0.258
6.25 0.263 0.259 0.261 0.129
3.12 0.178 0.172 0.175 0.043
0 0.131 0.133 0.132  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog HAMP ELISA Kit was designed for the quantitative measurement of Dog HAMP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

Customer Reviews and Q&A

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 Q&A
Q:

My name is Thitapa, student of veterinary medicine, Chulalongkorn university from Thailand.
Recently we are working on a study about canine hepcidin and I would like to know how much it is for 1 dog hepcidin ELISA test kit 96-well (I couldn't find it on the website ,only found the 24-T one) Moreover, if you have any evidence about any studies that your product had been used to evaluate hepcidin level please attach the information. We would be really appreciated.

A:
Thanks for your concern on Cusabio and your email!We can supply both size of Dog Hepcidin(HAMP) ELISA kit ,24T & 96T.
#CSB-EL010124DO,Dog Hepcidin(HAMP) ELISA kit -96T
Regarding this kit,we tested the normal dog serum ,plasma samples with 0 dilutions and we got the results falling in 12.5--100ng/ml.You can take this for your reference.As long as the content of the sample falls in the detection range of this kit,it can be detected out.And I will send you manual reference by email later.
If you are interested to order it,pls kindly contact and enquire local distributor.

Target Background

Function
(From Uniprot)
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.; Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
Gene References into Functions
  1. The objectives of this study were to clone and sequence the canine hepcidin gene and to gather preliminary data about tissue expression of hepcidin in dogs. PMID: 15570559
Subcellular Location
Secreted.
Protein Families
Hepcidin family
Database Links
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