Dog Interleukin 10,IL-10 ELISA Kit

Code CSB-E11261c
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P48411
Lead Time 3-5 working days
Abbreviation IL10
Target Name interleukin 10
Alias CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
HGNC 5962
RGD 2886
MGI 96537
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %97
Range %92-108
1:2Average %97
Range %91-105
1:4Average %89
Range %84-96
1:8Average %92
Range %87-98
Recovery
The recovery of dog IL-10spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-95
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
1002.954 2.967 2.961 2.853
502.388 2.398 2.393 2.285
251.758 1.786 1.772 1.664
12.51.035 1.109 1.072 0.964
6.250.502 0.546 0.524 0.416
3.120.271 0.267 0.269 0.161
1.560.184 0.196 0.190 0.082
00.112 0.103 0.108  
References
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Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location Secreted
Protein Families IL-10 family
Database Links

KEGG: cfa:403628

STRING: 9615.ENSCAFP00000016812

UniGene: Cfa.38

Pathway Jak-STAT signaling pathway
T cell receptor signaling pathway
FoxO signaling pathway

Q&A and Customer Reviews

Q&A
Q:

If using this kit it is necessary to perform a a blood clotting time of 2 hours or overnight at 4 ° C and 15 minutes of centrifugation, and if so what is the reason for this procedure.

A:
Thanks for your inquiry.
It is the sample preparation method for serum indeed.
It is fine to do as the you mentioned for serum. Here also attached our manual for your checking.
The sample preparation method is through our our experimental experience and get it to prepare relatively large amounts of serum samples with relatively simple components.Pls let me know if you have any further questions. Thank you.
Customer Reviews

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