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| Code | CSB-E11261c |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-108 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| The recovery of dog IL-10spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This dog IL10 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of dog IL10 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the IL10 levels in the sample and is measured at 450 nm through a microplate reader.
IL10 is an anti-inflammatory cytokine that is produced by many hematopoietic cells and mainly functions to inhibit macrophage and dendritic cell (DC) functions, which are necessary for the capture, control, and initiation of immune responses to pathogens. IL10 both impedes pathogen removal and improves immunopathology. IL-10 suppresses the expression of cytokines such as TNF-α, IL-1β, and IL-8, and can repress the expression of adhesion molecules. In addition, IL-10 can deactivate monocytes by downregulating the expression of MHC surface molecules. IL10 also plays a role in the control of allergy and asthma.
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If using this kit it is necessary to perform a a blood clotting time of 2 hours or overnight at 4 ° C and 15 minutes of centrifugation, and if so what is the reason for this procedure.
