Dog von Willebrand Factor,vWF ELISA Kit

Code CSB-E12759c
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
von Willebrand factor
Alternative Names
VWF ELISA Kit; F8VWF ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit
Abbreviation
VWF
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.32 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 101  
Range % 95-107  
1:200 Average % 94  
Range % 90-100  
1:400 Average % 98  
Range % 94-102  
1:800 Average % 88  
Range % 84-93  
Recovery
The recovery of dog vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 97 90-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
40 0.093 0.101 0.097    
20 0.209 0.216 0.213    
10 0.422 0.441 0.432    
5 0.739 0.715 0.727    
2.5 1.168 1.124 1.146    
1.25 1.602 1.709 1.656    
0.625 1.779 1.879 1.829    
0 2.170 2.297 2.234    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog VWF ELISA Kit was designed for the quantitative measurement of Dog VWF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.32 ng/mL.

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Target Background

Function
(From Uniprot)
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Gene References into Functions
  1. only the c.1657G variant was found in the homozygous state exclusively in Von Willebrand disease affected dogs, and this variant is the strongest candidate to be the cause of VWD type 2 in the German Wirehaired Pointer and German Shorthaired Pointer breeds PMID: 28696025
  2. the CD-31 and vonWillebrand factor (vWF) expressions and the cell morphological alterations ofarachnoid villus endothelium in different stages of animal hydrocephalus PMID: 17593818
  3. Expression of mutant VWF proteins confirmed that VWF multimerization is not necessary for Weibel-Palade body creation PMID: 15331450
Involvement in disease
Defects in VWF are the cause of von Willebrand disease (VWD) in the Scottish Terrier. VWD is characterized by frequent bleeding. Type I VWD is associated with a deficiency of VWF; type II by normal to decreased plasma level of VWF; type III by a virtual absence of VWF.
Subcellular Location
Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules.
Tissue Specificity
Plasma.
Database Links
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