Fish Acetylcholinesterase(AChE)ELISA Kit

Instructions
Code CSB-E17001Fh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Acetylcholinesterase(AChE)
Alternative Names acheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit
Abbreviation AChE
Uniprot No. P04058
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 2.5 ng/mL-40 ng/mL
Sensitivity 2.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 89-103
1:2 Average % 92
Range % 87-101
1:4 Average % 93
Range % 87-100
1:8 Average % 89
Range % 83-99
Recovery
The recovery of fish AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 84-93
EDTA plasma (n=4) 94 87-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average  
40 0.284 0.292 0.288  
20 0.521 0.532 0.527  
10 0.886 0.891 0.889  
5 1.374 1.382 1.378  
2.5 2.583 2.599 2.591  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
Subcellular Location Isoform H: Cell membrane, Lipid-anchor, GPI-anchor, Cell junction, synapse, SUBCELLULAR LOCATION: Isoform T: Cell membrane, Peripheral membrane protein, Cell junction, synapse
Protein Families Type-B carboxylesterase/lipase family
Tissue Specificity Found in the synapses and to a lower extent in extrajunctional areas of muscle and nerve, and on erythrocyte membranes.

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