Fish Cholecystokinin(CCK) ELISA Kit

Code CSB-E13054Fh
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. D7S9W7
Lead Time 3-5 working days
Abbreviation CCK
Target Name Cholecystokinin(CCK)
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 200 pg/mL-5000 pg/mL
Sensitivity 125 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %86-105
1:2Average %89
Range %84-96
1:4Average %96
Range %91-102
1:8Average %93
Range %86-105
Recovery
The recovery of fish CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8885-91
EDTA plasma (n=4)9586-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
50000.137 0.135 0.136
20000.241 0.228 0.235
12000.324 0.339 0.332
5000.616 0.577 0.597
2001.235 1.274 1.255
02.602 2.696 2.649
References
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Q&A and Customer Reviews

Q&A
Q:

Fish CCk ELISA kit cat.numb
Could you give me the specific size parameters of the plate wells? As she will need it for the plate reader machine.

A:
Thanks for your inquiry.
The kit size is 96T and it is 8wells× 12 strips.Pls check the size:
W:8.5cm
L:12.5cm
X:1.25cm
Y:1cm
Generally the microplate reader with 450nm wave can be used. Our company uses the reader from Thermo MK-3.Pls let me know if you have any further questions. Thank you.
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