Fish Cholecystokinin(CCK) ELISA Kit

Fish Cholecystokinin(CCK) ELISA Kit

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Fish Cholecystokinin(CCK) ELISA Kit

Specification

Code

CSB-E13054Fh

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

D7S9W7

Lead Time

3-5 working days

Abbreviation

CCK

Target Name

Cholecystokinin(CCK)

Species

Fish

Sample Types

serum, plasma, tissue homogenates

Detection Range

200 pg/mL-5000 pg/mL

Sensitivity

125 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-105
1:2	Average %	89
	Range %	84-96
1:4	Average %	96
	Range %	91-102
1:8	Average %	93
	Range %	86-105

Recovery

The recovery of fish CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-91
EDTA plasma (n=4)	95	86-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
5000	0.137	0.135	0.136
2000	0.241	0.228	0.235
1200	0.324	0.339	0.332
500	0.616	0.577	0.597
200	1.235	1.274	1.255
0	2.602	2.696	2.649

References

Effects of genetically modified and non-genetically modified soybeans with different heat treatments on growth and health of Cyprinidae species with different feeding habits Haokun Liu, et al,Aquaculture Research,2018
Effect of dietary phospholipid sources and levels on growth performance, enzymes activity, cholecystokinin and lipoprotein fractions of rainbow trout (Oncorhynchus mykiss) fry Hamid Mohamadi Azarm et al,Aquaculture Research,2013

Still Have Questions?

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Q&A

Fish CCk ELISA kit cat.numb
Could you give me the specific size parameters of the plate wells? As she will need it for the plate reader machine.

Thanks for your inquiry.
The kit size is 96T and it is 8wells× 12 strips.Pls check the size:
W：8.5cm
L：12.5cm
X：1.25cm
Y：1cm
Generally the microplate reader with 450nm wave can be used. Our company uses the reader from Thermo MK-3.Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.