Fish Cholecystokinin(CCK) ELISA Kit

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Fish Cholecystokinin(CCK) ELISA Kit

Specification

Code

CSB-E13054Fh

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

D7S9W7

Lead Time

3-5 working days

Abbreviation

CCK

Target Name

Cholecystokinin(CCK)

Species

Fish

Sample Types

serum, plasma, tissue homogenates

Detection Range

200 pg/mL-5000 pg/mL

Sensitivity

125 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-105
1:2	Average %	89
	Range %	84-96
1:4	Average %	96
	Range %	91-102
1:8	Average %	93
	Range %	86-105

Recovery

The recovery of fish CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-91
EDTA plasma (n=4)	95	86-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
5000	0.137	0.135	0.136
2000	0.241	0.228	0.235
1200	0.324	0.339	0.332
500	0.616	0.577	0.597
200	1.235	1.274	1.255
0	2.602	2.696	2.649

References

Effect of dietary phospholipid sources and levels on growth performance, enzymes activity, cholecystokinin and lipoprotein fractions of rainbow trout (Oncorhynchus mykiss) fry Hamid Mohamadi Azarm et al,Aquaculture Research,March 44(4): 634-644
Effects of genetically modified and non-genetically modified soybeans with different heat treatments on growth and health of Cyprinidae species with different feeding habits Haokun Liu, et al,Aquaculture Research,December 14

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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