Fish Cholecystokinin(CCK) ELISA Kit

Code CSB-E13054Fh
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. D7S9W7
Lead Time 3-5 working days
Abbreviation CCK
Target Name Cholecystokinin(CCK)
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 200 pg/mL-5000 pg/mL
Sensitivity 125 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of fish CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %95
Range %86-105
1:2Average %89
Range %84-96
1:4Average %96
Range %91-102
1:8Average %93
Range %86-105
The recovery of fish CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8885-91
EDTA plasma (n=4)9586-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
50000.137 0.135 0.136
20000.241 0.228 0.235
12000.324 0.339 0.332
5000.616 0.577 0.597
2001.235 1.274 1.255
02.602 2.696 2.649
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