Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

Instructions
Code CSB-EL006395FI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cytochrome P450, family 1, subfamily A, polypeptide 1
Alternative Names cyp1a1 ELISA kit; cyp1aCytochrome P450 1A1 ELISA kit; EC 1.14.14.1 ELISA kit; CYPIA1 ELISA kit
Abbreviation CYP1A1
Uniprot No. Q6JZS3
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 75 pg/mL-1200 pg/mL
Sensitivity 75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish CYP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-98  
1:2 Average % 100  
Range % 92-107  
1:4 Average % 85  
Range % 80-91  
1:8 Average % 88  
Range % 83-96  
Recovery
The recovery of fish CYP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 88-95  
EDTA plasma (n=4) 94 91-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1200 0.146 0.147 0.147    
600 0.238 0.254 0.246    
300 0.524 0.553 0.539    
150 1.112 1.135 1.124    
75 2.338 2.277 2.308    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Citations

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Target Data

Function Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Subcellular Location Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane, Peripheral membrane protein
Protein Families Cytochrome P450 family
Database Links

STRING: 8090.ENSORLP00000018073

UniGene: Ola.6266

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