Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

Code CSB-EL006395FI
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
cytochrome P450, family 1, subfamily A, polypeptide 1
Alternative Names
cyp1a1 ELISA kit; cyp1aCytochrome P450 1A1 ELISA kit; EC 1.14.14.1 ELISA kit; CYPIA1 ELISA kit
Abbreviation
CYP1A1
Uniprot No.
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
75 pg/mL-1200 pg/mL
Sensitivity
75 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish CYP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-98  
1:2 Average % 100  
Range % 92-107  
1:4 Average % 85  
Range % 80-91  
1:8 Average % 88  
Range % 83-96  
Recovery
The recovery of fish CYP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 88-95  
EDTA plasma (n=4) 94 91-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1200 0.146 0.147 0.147    
600 0.238 0.254 0.246    
300 0.524 0.553 0.539    
150 1.112 1.135 1.124    
75 2.338 2.277 2.308    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Fish CYP1A1 ELISA Kit was designed for the quantitative measurement of Fish CYP1A1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 75 pg/mL-1200 pg/mL and the sensitivity is 75 pg/mL.

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Target Background

Function
(From Uniprot)
Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Protein Families
Cytochrome P450 family
Database Links
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