Fish Insulin-like growth factor II(IGF2) ELISA kit

Code CSB-EL011088FI
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
insulin-like growth factor 2 (somatomedin A)
Alternative Names
igf2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin ELISA Kit
Abbreviation
IGF2
Uniprot No.
Species
Fish
Sample Types
serum, plasma
Detection Range
62.5 pg/mL-1000 pg/mL
Sensitivity
31.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 91
Range % 87-102
1:200 Average % 95
Range % 91-99
1:400 Average % 96
Range % 88-106
1:800 Average % 92
Range % 88-96
Recovery
The recovery of fish IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 90-102
EDTA plasma (n=4) 93 85-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average  
1000 0.085 0.089 0.087  
500 0.192 0.205 0.199  
250 0.485 0.489 0.487  
125 0.942 0.937 0.940  
62.5 1.613 1.572 1.593  
0 2.595 2.624 2.610  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Fish IGF2 ELISA Kit was designed for the quantitative measurement of Fish IGF2 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-1000 pg/mL and the sensitivity is 31.25 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for integrin which is required for IGF2 signaling.
Subcellular Location
Secreted.
Protein Families
Insulin family
Database Links

UniGene: Omy.11849

CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1