Fish Interleukin 6,IL-6 ELISA Kit

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Fish Interleukin 6,IL-6 ELISA Kit

Name: Fish Interleukin 6,IL-6 ELISA Kit
Brand: CUSABIO
SKU: CSB-E13258Fh

^Instructions

Code	CSB-E13258Fh
Size	96T,5×96T,10×96T
Trial Size
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Target Background

Target Name

Interleukin 6,IL-6

Alternative Names

il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit

Abbreviation

IL-6

Uniprot No.

A0S0B0

Species

Fish

Sample Types

serum, plasma, tissue homogenates

Detection Range

12.5 pg/mL-200 pg/mL

Sensitivity

12.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	89
	Range %	82-94
1:4	Average %	103
	Range %	94-107
1:8	Average %	96
	Range %	90-101

Recovery

The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	91-104
EDTA plasma (n=4)	91	84-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.128	0.133	0.131
100	0.269	0.258	0.264
50	0.449	0.431	0.440
25	0.851	0.842	0.847
12.5	1.813	1.783	1.798

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Transcriptome analysis of immune-related gene expression in Yellow River carp (Cyprinus carpio var.) after challenge with Flavobacterium columnare Y Jia,Microbial pathogenesis,2021
Dietary taurine modulates hepatic oxidative status, ER stress and inflammation in juvenile turbot (Scophthalmus maximus L.) fed high carbohydrate diets Yue Zhang,Fish & shellfish immunology,2020
Inclusion of condensed tannins in Lateolabrax japonicus diets: Effects on growth, nutrient digestibility, antioxidant and immune capacity and copper sulphate stress resistance K Peng,Aquaculture Reports,2020
The effects of four plant extracts on growth, sex reversal, immunological and haemato‐biochemical parameters in Nile tilapia, Oreochmomis niloticus (Linnaeus, 1758) I Ghosal,Aquaculture Research,2020
Quercetin affects shoaling and anxiety behaviors in zebrafish: Involvement of neuroinflammation and neuron apoptosis J Zhang,Fish & Shellfish Immunology,2020
Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
Administration of a Polyphenol-Enriched Feed to Farmed Sea Bass (Dicentrarchus labrax L.) Modulates Intestinal and Spleen Immune Responses. Magrone T.et al,Oxid Med Cell Longev.,2016
Cytokine Responses in Gills of Capoeta umbla as Biomarkers of Environmental Pollution Danabas D.et al,Water Environ Res.,2016
Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,2016
Fat-associated lymphoid cluster in Cyprinus carpio: Characterisation and its relation with peritoneal haemangiosarcoma Madera-Sandoval RL. et al,Fish Shellfish Immuno,2015

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Function (From Uniprot)	Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response (By similarity).
Subcellular Location	Secreted
Protein Families	IL-6 superfamily
Tissue Specificity	Expressed in kidney and spleen. Low expression in liver and gills.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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