Fish Interleukin 6,IL-6 ELISA Kit

Code CSB-E13258Fh
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Fish IL-6 ELISA Kit was designed for the quantitative measurement of Fish IL-6 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 12.5 pg/mL-200 pg/mL and the sensitivity is 12.5 pg/mL.

Target Name Interleukin 6,IL-6
Alternative Names il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
Abbreviation IL-6
Uniprot No. A0S0B0
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 pg/mL-200 pg/mL
Sensitivity 12.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 88-97  
1:2 Average % 89  
Range % 82-94  
1:4 Average % 103  
Range % 94-107  
1:8 Average % 96  
Range % 90-101  
Recovery
The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 91-104  
EDTA plasma (n=4) 91 84-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
200 0.128 0.133 0.131    
100 0.269 0.258 0.264    
50 0.449 0.431 0.440    
25 0.851 0.842 0.847    
12.5 1.813 1.783 1.798    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
Subcellular Location Secreted.
Protein Families IL-6 superfamily
Tissue Specificity Expressed in kidney and spleen. Low expression in liver and gills.
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