Target Name |
Interleukin 6,IL-6 |
Alternative Names |
il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit |
Abbreviation |
IL-6 |
Uniprot No. |
A0S0B0 |
Species |
Fish |
Sample Types |
serum, plasma, tissue homogenates |
Detection Range |
12.5 pg/mL-200 pg/mL |
Sensitivity |
12.5 pg/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Immunology
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Assay Principle |
quantitative |
Measurement |
Competitive |
Precision |
Intra-assay Precision (Precision within an assay): CV%<15% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<15% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
Sample |
Serum(n=4) |
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1:1 |
Average % |
93 |
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Range % |
88-97 |
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1:2 |
Average % |
89 |
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Range % |
82-94 |
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1:4 |
Average % |
103 |
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Range % |
94-107 |
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1:8 |
Average % |
96 |
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Range % |
90-101 |
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Recovery |
The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
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Serum (n=5) |
100 |
91-104 |
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EDTA plasma (n=4) |
91 |
84-98 |
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Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
pg/ml |
OD1 |
OD2 |
Average |
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200 |
0.128 |
0.133 |
0.131 |
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100 |
0.269 |
0.258 |
0.264 |
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50 |
0.449 |
0.431 |
0.440 |
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25 |
0.851 |
0.842 |
0.847 |
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12.5 |
1.813 |
1.783 |
1.798 |
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Troubleshooting
and FAQs |
ELISA kit FAQs |
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
3-5 working days |