Horse Interleukin 6(IL-6)ELISA Kit

Instructions
Code CSB-E16634Hs
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Interleukin 6(IL-6)
Alternative Names IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
Abbreviation IL-6
Uniprot No. Q95181
Species Equus caballus (Horse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of horse IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %84-98
1:2Average %90
Range %85-95
1:4Average %96
Range %92-101
1:8Average %104
Range %96-108
Recovery
The recovery of horse IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9388-97
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.267 2.235 2.251 2.136
1001.482 1.589 1.536 1.421
500.954 0.999 0.977 0.862
250.602 0.612 0.607 0.492
12.50.432 0.452 0.442 0.327
6.250.269 0.289 0.279 0.164
3.120.218 0.230 0.224 0.109
00.111 0.119 0.115  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).
Gene References into Functions
  1. These data suggest that IL-6 may play a key role in equine metabolic syndrome (EMS), and that pro-inflammatory cytokines levels in serum may serve as an additional tool for diagnosing EMS. PMID: 25269712
  2. IL-6 stimulation decreased chondrocyte expression of the canonical Wnt signaling pathway transactivator beta-catenin, induced expression of inhibitors of the Wnt pathway, and increased expression of GDF-5. PMID: 24471749
  3. study shows that IL-6 is rapidly induced in BAL-cells of airway-compromised horses in response to adenosine exposure, probably through A2BAR activation and that this effect can be modulated by A2AAR PMID: 21496101
  4. Our data show the presence of a polymorphism downstream of the equine IL-6 gene that is associated with the basal Cu:Zn ratio in horses independent of breed. PMID: 21330167
  5. Expression levels of IL-6 are significantly increased in peripheral blood mononuclear cells from trained horses compared to sedentary animals. PMID: 21255427
  6. Failure of passive transfer may directly influence the serum IL-6 concentration in septic foals. Neither serum IL-6 nor IL-10 alone, were useful diagnostic indices of sepsis in equine neonates. PMID: 19501415
  7. The contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of IL-6, IL-10 and TNF-alpha, in horses with recurrent airway obstruction during exacerbation and in remission is reported. PMID: 18294392
  8. The effects of semen extender and seminal plasma on the expression of inflammatory modulators in the endometrium of mares are reported. PMID: 18584861

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Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

KEGG: ecb:100034196

STRING: 9796.ENSECAP00000014219

UniGene: Eca.12956

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  Email: [email protected]
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