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Fish Interleukin-8,IL-8 ELISA Kit

Citations (2) Protocol
Code CSB-E12877Fh
Size 96T,5×96T,10×96T
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Product Details

Abbreviation
IL8/CXCL8
Species
Fish
Sample Types
serum, plasma
Detection Range
31.25 pg/mL-500 pg/mL
Sensitivity
31.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 93    
Range % 92-97    
1:2 Average % 95    
Range % 85-100    
1:4 Average % 93    
Range % 89-101    
1:8 Average % 95    
Range % 86-100    
Recovery
The recovery of fish IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 96 89-100    
EDTA plasma (n=4) 95 85-105    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average 　    
500 0.172 0.176 0.174 　    
250 0.392 0.399 0.396 　    
125 0.635 0.628 0.632 　    
62.5 1.038 1.046 1.042 　    
31.25 2.456 2.417 2.437 　    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Fish IL8/CXCL8 ELISA Kit was designed for the quantitative measurement of Fish IL8/CXCL8 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 31.25 pg/mL-500 pg/mL and the sensitivity is 31.25 pg/mL.

Citations

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