Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Code CSB-E12695Fh
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Prolactin/Luteotropic Hormone(PRL/LTH)
Alternative Names
prl1 ELISA Kit; Prolactin-1 ELISA Kit; PRL-188 ELISA Kit; Prolactin I ELISA Kit
Abbreviation
PRL/LTH
Uniprot No.
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
10 μIU/mL-4000 μIU/mL
Sensitivity
10 μIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 91-100  
1:2 Average % 95  
Range % 86-104  
1:4 Average % 90  
Range % 85-95  
1:8 Average % 96  
Range % 92-108  
Recovery
The recovery of fish PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 91-102  
EDTA plasma (n=4) 95 88-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average    
4000 0.350 0.364 0.357    
750 0.697 0.705 0.701    
125 1.196 1.203 1.200    
35 1.523 1.519 1.521    
10 1.700 1.707 1.704    
0 2.244 2.242 2.243    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Fish PRL/LTH ELISA Kit was designed for the quantitative measurement of Fish PRL/LTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 10 μIU/mL-4000 μIU/mL and the sensitivity is 10 μIU/mL.

Customer Reviews and Q&A

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 Q&A
Q:

Which fish species / family is it optimized for? We work with a non-model species and would like to check its reactivity. Thank you.

A:
Thanks for your inquiry.
CSB-E12695Fh Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit
This kit is designed against salmon. There is no requirement for fish species. Gnerally all kinds of fish can be detected out. We suggest you do a pretest.
Pls let me know if you have any further questions. Thank you.

Target Background

Subcellular Location
Secreted.
Protein Families
Somatotropin/prolactin family
Database Links

UniGene: Oni.44217

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