Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Instructions
Code CSB-E12695Fh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Prolactin/Luteotropic Hormone(PRL/LTH)
Alternative Names prl1 ELISA Kit; Prolactin-1 ELISA Kit; PRL-188 ELISA Kit; Prolactin I ELISA Kit
Abbreviation PRL/LTH
Uniprot No. P09319
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 10 μIU/mL-4000 μIU/mL
Sensitivity 10 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 91-100  
1:2 Average % 95  
Range % 86-104  
1:4 Average % 90  
Range % 85-95  
1:8 Average % 96  
Range % 92-108  
Recovery
The recovery of fish PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 91-102  
EDTA plasma (n=4) 95 88-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average    
4000 0.350 0.364 0.357    
750 0.697 0.705 0.701    
125 1.196 1.203 1.200    
35 1.523 1.519 1.521    
10 1.700 1.707 1.704    
0 2.244 2.242 2.243    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Which fish species / family is it optimized for? We work with a non-model species and would like to check its reactivity. Thank you.

A:
Thanks for your inquiry.
CSB-E12695Fh Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit
This kit is designed against salmon. There is no requirement for fish species. Gnerally all kinds of fish can be detected out. We suggest you do a pretest.
Pls let me know if you have any further questions. Thank you.

Target Background

Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

UniGene: Oni.44217

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