Fish gonadotropin-releasing hormone,GnRH ELISA Kit

Instructions
Code CSB-E08810f
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name gonadotropin-releasing hormone,GnRH
Abbreviation GnRH
Uniprot No. Q5Y835
Species Fish
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 0.5 ng/mL-20 ng/mL
Sensitivity 0.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 93-104  
1:2 Average % 94  
Range % 85-103  
1:4 Average % 95  
Range % 83-102  
1:8 Average % 92  
Range % 87-101  
Recovery
The recovery of fish GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-94  
EDTA plasma (n=4) 94 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
20 0.134 0.140 0.137    
10 0.232 0.242 0.237    
4.6 0.398 0.409 0.404    
1.5 0.714 0.687 0.701    
0.5 1.293 1.266 1.280    
0 2.711 2.740 2.726    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

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 Q&A
Q:

Fish have over a dozen different forms of GnRHs. I would llike to know for which fish GnRH form is this assay designed before placing an order.

A:
Thanks for your inquiry.
This kit is designed against Zebrafish. Pls refer to: http://www.uniprot.org/uniprot/Q5Y835
Q:

If the gene mentioned in the data is indeed gnrh2, the assay cannot be specific for zebrafish or any other fish species. All vertebrate animals, including humans, have gnrh2 gene with mostly unknown function. The GnRH of interest in zebrafish is salmon GnRH or gnrh3 gene. I have to think about it. This kit may not be any better for zebrafish than those for mammalian GnRH because gnrh2 gene encodes for exactly the same functional peptide in all vertebrates.

Do you have data which shows this kit is specific to zebrafish? Could you perhaps send the COA?

A:
Thanks for your inquiry.
GnRH has different isoforms:GnRH,GnRH2 and GnRH3. GnRH-2 is very conservative in different species of fish.
Pls kindly refer to: https://www.nature.com/articles/srep03676.
CSB-E08810f is designed against zebrafish. We tested undiluted serum and plasma samples before and the test value is 0.5--1.5ng/ml.
I will send you COA by email.
Pls let me know if you have any further questions. Thank you.
Q:

Thanks for your response. Based on the literature you provided it seems GnRH2 is not only conserved among fish species but among all vertebrates.
Since I am curious what the difference is between this kit and your kits from other species. Since the antigen is so highly conserved couldn't I use any of your other GnRH kits instead?

A:
Although GnRH2 is highly conserved, different types of animals have different matrix.
Our kit for fish is designed against fish. For different animals, the raw materials used in the kits are different.

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