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| Code | CSB-E08810f |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of fish GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 85-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 83-102 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-101 | |||||
| The recovery of fish GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 94 | 89-98 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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This Fish GnRH ELISA Kit was designed for the quantitative measurement of Fish GnRH protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Competitive ELISA kit, its detection range is 0.5 ng/mL-20 ng/mL and the sensitivity is 0.5 ng/mL.
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Fish have over a dozen different forms of GnRHs. I would llike to know for which fish GnRH form is this assay designed before placing an order.
If the gene mentioned in the data is indeed gnrh2, the assay cannot be specific for zebrafish or any other fish species. All vertebrate animals, including humans, have gnrh2 gene with mostly unknown function. The GnRH of interest in zebrafish is salmon GnRH or gnrh3 gene. I have to think about it. This kit may not be any better for zebrafish than those for mammalian GnRH because gnrh2 gene encodes for exactly the same functional peptide in all vertebrates.
Do you have data which shows this kit is specific to zebrafish? Could you perhaps send the COA?
Thanks for your response. Based on the literature you provided it seems GnRH2 is not only conserved among fish species but among all vertebrates.
Since I am curious what the difference is between this kit and your kits from other species. Since the antigen is so highly conserved couldn't I use any of your other GnRH kits instead?
