Fish lysozyme (renal amyloidosis) (LZM) ELISA kit

Instructions
Code CSB-E17296Fh
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lysozyme (renal amyloidosis) (LZM)
Abbreviation LZM
Species Fish
Sample Types serum, plasma
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 93  
Range % 85-100  
1:40 Average % 91  
Range % 86-99  
1:80 Average % 95  
Range % 90-103  
1:160 Average % 95  
Range % 90-98  
Recovery
The recovery of fish LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-94  
EDTA plasma (n=4) 93 85-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
200 0.088 0.090 0.089    
100 0.206 0.194 0.200    
50 0.307 0.300 0.304    
25 0.418 0.423 0.421    
12.5 0.627 0.605 0.616    
6.25 1.045 1.031 1.038    
3.12 1.648 1.524 1.586    
0 2.685 2.657 2.671    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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