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Goat Interleukin 10,IL-10 ELISA Kit

Instructions
Code CSB-E09809g
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Interleukin 10,IL-10
Abbreviation IL10
Uniprot No. A7Y3X5
Species Capra hircus (Goat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 82  
Range % 80-85  
1:2 Average % 102  
Range % 97-107  
1:4 Average % 89  
Range % 84-93  
1:8 Average % 107  
Range % 103-111  
Recovery
The recovery of goat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 85 80-90  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.725 2.768 2.747 2.615  
1000 2.059 2.000 2.030 1.898  
500 1.321 1.368 1.345 1.213  
250 0.741 0.758 0.750 0.618  
125 0.455 0.432 0.444 0.312  
62.5 0.307 0.301 0.304 0.172  
31.25 0.228 0.226 0.227 0.095  
0 0.131 0.133 0.132    
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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