Vitellogenin is a phospholipoglycoprotein precursor to egg yolk proteins that serves as an important biomarker in fish reproductive biology and endocrine research. Female fish synthesize this protein in the liver under estrogen stimulation. The protein then travels through the bloodstream to developing oocytes, where it becomes incorporated as yolk proteins necessary for embryonic development. Researchers commonly use VTG levels in aquaculture and environmental toxicology studies to measure reproductive status, estrogenic exposure, and endocrine disruption in fish populations.
The Grouper Vitellogenin ELISA Kit (CSB-E14116Fh) provides quantitative measurement of VTG in grouper samples using a competitive assay principle. This kit works with serum, plasma, and tissue homogenates with a detection range of 50 ng/mL to 2000 ng/mL and a sensitivity of 50 ng/mL. The assay requires 50-100 μL sample volume, operates with detection at 450 nm wavelength, and can be completed within 1-5 hours.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
Researchers have applied this ELISA kit in environmental toxicology research to study endocrine disruption effects in aquatic organisms. The kit works as a tool for detecting vitellogenin as a biomarker of endocrine system disruption caused by xenoestrogenic compounds in fish species.
• Endocrine disruption studies: Measuring vitellogenin levels in fish exposed to environmental contaminants to evaluate endocrine disrupting effects of xenoestrogens
• Environmental toxicology: Studying biomarker responses in aquatic organisms collected from contaminated versus non-contaminated water bodies
• Biomarker validation: Confirming and quantifying vitellogenin expression in liver and plasma samples as an indicator of early-stage endocrine disruption
• Comparative exposure studies: Analyzing vitellogenin secretion patterns between control organisms and those exposed to industrial pollutants in both natural and semi-controlled environments
