Grouper Vitellogenin,VTG ELISA Kit

Code CSB-E14116Fh
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Grouper VTG ELISA Kit was designed for the quantitative measurement of Grouper VTG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 50 ng/mL-2000 ng/mL and the sensitivity is 50 ng/mL.

Alternative Names vtg1 ELISA Kit; Vitellogenin ELISA Kit; VTG) [Cleaved into: Lipovitellin I ELISA Kit; LVI); Phosvitin ELISA Kit; PV); Lipovitellin II ELISA Kit; LVII)] ELISA Kit
Abbreviation VTG
Uniprot No. Q5IGP8
Species Grouper
Sample Types serum, plasma, tissue homogenates
Detection Range 50 ng/mL-2000 ng/mL
Sensitivity 50 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of grouper VTG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 84-97  
1:2 Average % 97  
Range % 91-102  
1:4 Average % 95  
Range % 88-103  
1:8 Average % 96  
Range % 91-101  
Recovery
The recovery of grouper VTG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-100  
EDTA plasma (n=4) 95 91-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
2000 0.174 0.175 0.175    
1000 0.359 0.378 0.369    
460 0.567 0.552 0.560    
150 0.872 0.891 0.882    
50 1.513 1.476 1.495    
0 2.389 2.411 2.400    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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