Target Name |
interleukin 10 |
Alternative Names |
IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit |
Abbreviation |
IL10 |
Uniprot No. |
Q9Z1Y5 |
Species |
Cavia porcellus (Guinea pig) |
Sample Types |
serum, plasma, tissue homogenates |
Detection Range |
31.25 pg/mL-2000 pg/mL |
Sensitivity |
7.81 pg/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Immunology
|
Assay Principle |
quantitative |
Measurement |
Sandwich |
Precision |
Intra-assay Precision (Precision within an assay): CV%<8% | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | Inter-assay Precision (Precision between assays): CV%<10% | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | |
|
Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | Sample | Serum(n=4) | | 1:1 | Average % | 90 | | Range % | 85-95 | | 1:2 | Average % | 96 | | Range % | 91-103 | | 1:4 | Average % | 95 | | Range % | 89-100 | | 1:8 | Average % | 97 | | Range % | 91-100 | |
|
Recovery |
The recovery of guinea pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
Sample Type | Average % Recovery | Range | | Serum (n=5) | 96 | 89-101 | | EDTA plasma (n=4) | 97 | 91-105 | | | | | | | | | | | | | | | |
|
Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
pg/ml | OD1 | OD2 | Average | Corrected | | 2000 | 2.894 | 2.903 | 2.899 | 2.780 | | 1000 | 2.107 | 2.115 | 2.111 | 1.992 | | 500 | 1.233 | 1.241 | 1.237 | 1.118 | | 250 | 0.756 | 0.767 | 0.762 | 0.643 | | 125 | 0.443 | 0.451 | 0.447 | 0.328 | | 62.5 | 0.289 | 0.299 | 0.294 | 0.175 | | 31.25 | 0.184 | 0.197 | 0.191 | 0.072 | | 0 | 0.118 | 0.120 | 0.119 | | |
|
|
Troubleshooting
and FAQs |
ELISA kit FAQs |
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
3-5 working days |