Guinea pig interleukin 10,IL-10 ELISA Kit

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Target Data

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Guinea pig interleukin 10,IL-10 ELISA Kit

Name: Guinea pig interleukin 10,IL-10 ELISA Kit
Brand: CUSABIO
SKU: CSB-E12915Gp

^Instructions

Code	CSB-E12915Gp
Size	96T,5×96T,10×96T
Trial Size
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Product Details
Citations
Customer Reviews and Q&A
Target Data

Target Name

interleukin 10

Alternative Names

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit

Abbreviation

IL10

Uniprot No.

Q9Z1Y5

Species

Cavia porcellus (Guinea pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	96
	Range %	91-103
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	91-100

Recovery

The recovery of guinea pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-101
EDTA plasma (n=4)	97	91-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.894	2.903	2.899	2.780
1000	2.107	2.115	2.111	1.992
500	1.233	1.241	1.237	1.118
250	0.756	0.767	0.762	0.643
125	0.443	0.451	0.447	0.328
62.5	0.289	0.299	0.294	0.175
31.25	0.184	0.197	0.191	0.072
0	0.118	0.120	0.119

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Isoleucine attenuates infection induced by E. coli challenge through modulation of intestinal endogenous antimicrobial peptide expression and inhibition of the increase of plasma endotoxin and IL-6 in weaned pigs Man Ren,Food & Function,2019
Dietary xylo-oligosaccharide improves intestinal function in weaned piglets Jie Yin, et al,Food & Function,2019

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Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location	Secreted
Protein Families	IL-10 family

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Related pathways

Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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