| Description |
This Guinea pig IL-4 ELISA Kit was designed for the quantitative measurement of Guinea pig IL-4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200pg/mL and the sensitivity is 0.78 pg/mL . |
| Target Name |
interleukin 4,IL-4 |
| Abbreviation |
IL-4 |
| Uniprot No. |
G0WM66 |
| Species |
Cavia porcellus (Guinea pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.12 pg/mL-200pg/mL |
| Sensitivity |
0.78 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 97 | | | Range % | 93-105 | | | 1:2 | Average % | 96 | | | Range % | 92-102 | | | 1:4 | Average % | 103 | | | Range % | 97-107 | | | 1:8 | Average % | 84 | | | Range % | 80-88 | |
|
| Recovery |
| The recovery of guinea pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 94 | 89-98 | | | EDTA plasma (n=4) | 95 | 90-100 | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.375 | 2.294 | 2.335 | 2.160 | | | 100 | 1.903 | 1.802 | 1.853 | 1.678 | | | 50 | 1.355 | 1.376 | 1.366 | 1.191 | | | 25 | 0.903 | 0.914 | 0.909 | 0.734 | | | 12.5 | 0.650 | 0.641 | 0.646 | 0.471 | | | 6.25 | 0.423 | 0.410 | 0.417 | 0.242 | | | 3.12 | 0.317 | 0.312 | 0.315 | 0.140 | | | 0 | 0.176 | 0.173 | 0.175 | | |
|
|
| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
