Guinea pig interleukin 4,IL-4 ELISA Kit

Code CSB-E10126Gu
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Guinea pig IL-4 ELISA Kit was designed for the quantitative measurement of Guinea pig IL-4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200pg/mL and the sensitivity is 0.78 pg/mL .

Target Name interleukin 4,IL-4
Abbreviation IL-4
Uniprot No. G0WM66
Species Cavia porcellus (Guinea pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %97
Range %93-105
1:2Average %96
Range %92-102
1:4Average %103
Range %97-107
1:8Average %84
Range %80-88
The recovery of guinea pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
2002.375 2.294 2.335 2.160
1001.903 1.802 1.853 1.678
501.355 1.376 1.366 1.191
250.903 0.914 0.909 0.734
12.50.650 0.641 0.646 0.471
6.250.423 0.410 0.417 0.242
3.120.317 0.312 0.315 0.140
00.176 0.173 0.175  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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