Hamster C-reactive protein(CRP) ELISA Kit

Code CSB-EL005991HA
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
C-reactive protein(CRP)
Alternative Names
CRP ELISA kit; PTX1C-reactive protein ELISA kit
Abbreviation
CRP
Uniprot No.
Species
Mesocricetus auratus (Golden hamster)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 ng/mL-800 ng/mL
Sensitivity
3.12 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of hamster CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %91
Range %85-96
1:10Average %87
Range %82-92
1:20Average %96
Range %89-103
1:40Average %98
Range %94-104
Recovery
The recovery of hamster CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8885-93
EDTA plasma (n=4)9687-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
8002.017 2.075 2.046 1.888
4001.592 1.584 1.588 1.430
2001.062 1.036 1.049 0.891
1000.625 0.586 0.606 0.448
500.456 0.441 0.449 0.291
250.309 0.293 0.301 0.143
12.50.231 0.218 0.225 0.067
00.155 0.161 0.158
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Hamster CRP ELISA Kit was designed for the quantitative measurement of Hamster CRP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

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Target Background

Function
(From Uniprot)
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Subcellular Location
Secreted.
Protein Families
Pentraxin family
Tissue Specificity
Found in plasma.
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