Hamster C-reactive protein(CRP) ELISA Kit

Code CSB-EL005991HA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name C-reactive protein(CRP)
Alternative Names CRP ELISA kit; PTX1C-reactive protein ELISA kit
Abbreviation CRP
Uniprot No. P49262
Species Mesocricetus auratus (Golden hamster)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of hamster CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:5Average %91
Range %85-96
1:10Average %87
Range %82-92
1:20Average %96
Range %89-103
1:40Average %98
Range %94-104
The recovery of hamster CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8885-93
EDTA plasma (n=4)9687-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
8002.017 2.075 2.046 1.888
4001.592 1.584 1.588 1.430
2001.062 1.036 1.049 0.891
1000.625 0.586 0.606 0.448
500.456 0.441 0.449 0.291
250.309 0.293 0.301 0.143
12.50.231 0.218 0.225 0.067
00.155 0.161 0.158
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Subcellular Location Secreted.
Protein Families Pentraxin family
Tissue Specificity Found in plasma.
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